Profiling of the transcriptome of Porphyra yezoensis with Solexa sequencing technology  

作　　者：YANG Hui MAO YunXiang KONG FanNa YANG GuanPin MA Fei WANG Li 

机构地区：[1]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China

出　　处：《Chinese Science Bulletin》2011年第20期2119-2130,共12页

基　　金：supported by the National Natural Science Foundation of China(30972247,30700621);Special Fund for Agro-scientific Research in the Public Interest(200903030);National High Technology Research and Development Program of China(2006AA10A402,2006AA10A413);Program for New Century Excellent Talents in University(NCET-06-0596);Promotive Research Fund for Young and Middle-aged Scientisits of Shandong Province(2008BS06002)

摘　　要：With high-throughput Solexa sequencing technology,we profiled Porphyra yezoensis transcriptomes from 8 different samples. More than 1200 megabases from 13333334 quality paired-end reads were generated,which were assembled into 31538 unigenes. Blast analysis showed that 56.7%unigenes were novel,which represented the specific genes of Porphyra and/or rhodophytes. Several hundreds of unigenes related to stress tolerance were discovered,including genes related to desiccation-(211) and high light-tolerance(31) ,flavonoid biosynthesis(10) ,reactive oxygen scavenging(48) and other stress-tolerance processes(208) ,which indicated there existed complex and diversity modes of stress tolerance in this species.A complete set of essential genes involved in the C3-(57) and C4-(44) carbon fixation pathway(except pyruvate phosphate dikinase) were discovered,which not only proved that they were actively transcribed but also clearly outlined the panoptic view of carbon fixation in Porphyra.Moreover,by statistically analyzing the types,proportions and frequencies of the interspersed repeats(TEs) and simple sequence repeats(SSRs) ,we discovered that the top three types of TEs were all retrotransposons and the trinucleotide was the absolute predominant type among SSRs,promoting our understanding of structural characteristics of the transcriptome.This study substantially improved the global view of the Porphyra genome and provided a valuable resource for future research.With high-throughput Solexa sequencing technology,we profiled Porphyra yezoensis transcriptomes from 8 different samples. More than 1200 megabases from 13333334 quality paired-end reads were generated,which were assembled into 31538 unigenes. Blast analysis showed that 56.7%unigenes were novel,which represented the specific genes of Porphyra and/or rhodophytes. Several hundreds of unigenes related to stress tolerance were discovered,including genes related to desiccation-（211） and high light-tolerance（31） ,flavonoid biosynthesis（10） ,reactive oxygen scavenging（48） and other stress-tolerance processes（208） ,which indicated there existed complex and diversity modes of stress tolerance in this species.A complete set of essential genes involved in the C3-（57） and C4-（44） carbon fixation pathway（except pyruvate phosphate dikinase） were discovered,which not only proved that they were actively transcribed but also clearly outlined the panoptic view of carbon fixation in Porphyra.Moreover,by statistically analyzing the types,proportions and frequencies of the interspersed repeats（TEs） and simple sequence repeats（SSRs） ,we discovered that the top three types of TEs were all retrotransposons and the trinucleotide was the absolute predominant type among SSRs,promoting our understanding of structural characteristics of the transcriptome.This study substantially improved the global view of the Porphyra genome and provided a valuable resource for future research.

关 键 词：反转录转座子 条斑紫菜 测序技术 技术分析 黄酮类化合物 相关基因 简单重复序列 SSRS 

分 类 号：S968.431[农业科学—水产养殖]