噬菌体展示技术在毒素检测分析中的应用  

The Usage of Phage Display Technique in Mycotoxins Detection

作　　者：胡骁飞[1] 栗力[2] 邢广旭[1] 裴亚峰[1] 职爱民[1] 王耀[1] 孙亚宁[1] 王磊[1] 赵丽娜[1] 邓瑞广[1] 

机构地区：[1]河南省农业科学院农业部动物免疫学重点实验室/河南省动物免疫学重点实验室,河南郑州450002 [2]河南省农村能源环境保护总站,河南郑州450002

出　　处：《河南农业科学》2011年第6期32-35,共4页Journal of Henan Agricultural Sciences

基　　金：河南省基础与前沿科研项目(092300410028)

摘　　要：霉菌毒素严重危害动物及人类健康,毒素检测已成为评价农产品、食品、饲料品质的重要内容之一。毒素检测有一定危险性,建立新的毒素检测手段意义重大。噬菌体展示技术是一种设计精巧、具有极强分离多肽功能的生物学技术。综述了此项技术的发展和在筛选霉菌毒素模拟表位肽中的作用及模拟表位肽在毒素检测中的应用,并展望了该技术的应用前景。Mycotoxins are serviously harmfull to animal and human kinds,which have been one of the key indexes of quality monitor of food,agricultural commodities,feedstuffs.It is peril for checker in the detection of mycotoxins,and it is necessary to develop a new method for mycotoxins determination safely.Phage display is a exquisite biotechnology,which has a great capacity for segregating peptide.The development and function in screening mimicking epitope of mycotoxins of phage display and the usage of mimicking epitope in mycotoxins detection were reviewed,and the future of the mimicking epitope was looked into.

关 键 词：霉菌毒素 检测 噬菌体展示技术 模拟表位肽 

分 类 号：S859.84[农业科学—临床兽医学]