酸性脲酶的固定化研究  

Immobilization of Acid Urease

作　　者：李慧娟[1] 曹智琨[1] 高军[1] 余建勇[1] 刘国华[1] 

机构地区：[1]山东科技大学化学与环境工程学院,山东青岛266510

出　　处：《化学工业与工程》2011年第4期50-54,共5页Chemical Industry and Engineering

基　　金：山东科技大学"春蕾计划"项目(2008AZZ074)

摘　　要：以明胶为包埋材料,戊二醛为交联剂固定化粪产碱菌所产的酸性脲酶。对酸性脲酶的固定化条件(包括明胶含量、戊二醛浓度、吸附时间、交联时间和粗酶液用量)及酶学性质(温度和pH值)进行了研究。结果表明,固定化酶的最适宜条件为:明胶的质量分数15%,戊二醛质量分数0.3%,吸附时间4 h,交联时间20 min,粗酶液用量4 mL。比较固定化酶与游离酶的酶学性质,结果表明:固定化酶的最适温度为50℃,游离酶的为30℃;固定化酶的最适pH值为4.0,游离酶的最适pH值为5.0。固定化酸性脲酶比游离酶更适于处理工业上所产生的尿素废液。Acid urease of Alcaligenes faecalis was immobilized onto the gelatin crosslinked with glutaraldehyde.Immobilization conditions and enzymatic properties of acid urease were determined.The optimum conditions of immobilized urease were as follows： gelatin content 15%,glutaraldehyde volume fraction 0.3%,absorption time 4 h,crosslinking time 20 min,crude enzyme volume 4 mL.Enzymatic characterizations of immobilized and free urease showed that the optimum temperature was 50 ℃ and 30 ℃,the optimum pH value was pH 4.0 and pH 5.0,respectively.Compared with free enzyme,the immobilized urease was more fit for dealing with urea waste water.

关 键 词：酸性脲酶 明胶 戊二醛 固定化 

分 类 号：TQ920[轻工技术与工程—发酵工程]