辐射后淋巴细胞的G显带染色体畸变分析  

G-banding chromosomal aberration assay of lymphocyte after radiation

作　　者：赵明刚[1,2] 施常备 赵华 袁勇 姚俊涛 陆建荣 袁彬 陈葳[1] 王翔[1] 

机构地区：[1]西安交通大学第一附属医院分子医学实验室,陕西西安710061 [2]陕西省肿瘤医院,陕西西安710061

出　　处：《现代肿瘤医学》2011年第7期1310-1312,共3页Journal of Modern Oncology

基　　金：陕西省卫生厅科研基金项目(编号2010D10)

摘　　要：目的:通过染色体G显带技术研究辐射后染色体的结构畸变情况。方法:4MV的X线辐射离体入血,淋巴细胞培养后,应用染色体G显带技术,分析辐射后染色体的结构畸变情况,包括双着丝粒、缺失、易位、环状染色体、染色体片段,等。结果:辐射后观察分散良好、形态清晰的细胞核型100个,染色体畸变总数为428,其中双着丝粒、缺失、易位、环状染色体、染色体片段的发生数分别为86、41、111、9、181。分析各号染色体辐射后畸变情况,未见明显差异。结论:染色体G带分析可以提供较丰富的染色体结构畸变信息。Objective：To validate the value of G-banding chromosomal aberration of lymphocyte after radiation.Methods： The blood was irradiated by 4 MV X-ray and was cultured 3 days.The G-banding chromosomal aberration was assayed including dicentrics,deletions,translocations,rings,and fragments chromosomal aberration.Results：There were 100 good karyotypes observed.The total of chromosomal aberration was 428.The dicentric,deletion,translocation,ring,and fragment chromosomal aberration were 86,41,111,9,and 181 respectively.There was no significant difference in chromosomal aberration after radiation in every NO.chromosome.Conclusion：The G-banding chromosomal aberration assay should be paid more attention in radiation biology.

关 键 词：辐射 染色体畸变 G显带 

分 类 号：R730.3[医药卫生—肿瘤]