乳杆菌对牛乳β-乳球蛋白致敏小鼠淋巴细胞分泌Th1/Th2型细胞因子和抗体的体外影响  

Effect of lactobacilli on production of Th1/Th2 cytokine and antibody in lymphocyte of bovine β-lactoglobulin-sensitized mice in vitro

作　　者：马冬雪[1] 孟祥晨[1] 高学军[1] 李艾黎[2] 徐渐[2] 卲红 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]东北农业大学兽医学博士后流动站,黑龙江哈尔滨150030

出　　处：《微生物学通报》2011年第7期1063-1069,共7页Microbiology China

基　　金：黑龙江省教育厅科学基金项目(No.12511052);中国博士后科学基金项目(No.20090460872)

摘　　要：为了分析乳杆菌对致敏小鼠脾淋巴细胞分泌Th1/Th2细胞因子及抗体的体外影响,用牛乳β-乳球蛋白腹腔注射BALB/c小鼠建立过敏症模型,造模成功后,分离致敏小鼠的脾淋巴细胞并与4种活/死乳杆菌(107 CFU/mL)体外共同孵育,ELISA法检测细胞上清液中细胞因子(IL-12、IFN-γ和IL-4)和抗体(总IgE、β-Lg特异性IgE和总IgG)含量。4种活/死乳杆菌均可体外调节致敏小鼠脾淋巴细胞分泌细胞因子和抗体的水平,特别是热致死的发酵乳杆菌和嗜酸乳杆菌可提高淋巴细胞IL-12和IFN-γ的分泌,抑制IL-4的分泌,使其IFN-γ/IL-4比值(代表Th1/Th2细胞平衡)高于活菌,与空白对照组比较差异显著(P<0.05)。同时,这两株热致死菌还可显著下调细胞上清液中总IgE、特异性IgE和总IgG抗体的浓度(P<0.05)。试验结果表明乳杆菌可提高牛乳β-乳球蛋白致敏小鼠脾淋巴细胞的IFN-γ/IL-4比值,进而纠正Th2占优势的Th1/Th2失衡,下调抗体分泌量,且具有菌株特异性。To investigate the effect of lactobacilli on the Th1/Th2 cytokine and antibody production in sen-sitized-mice spleen lymphocytes in vitro,BALB/c mice were intraperitoneally injected with β-lactoglobulin,After successfully established the allergic model,the sensitized splenic cells were stimulated with ac-tive/heat-killed lactobacilli（107 CFU/mL） at different species in vitro.Supernatants were collected to measure the cytokines（IL-12,IFN-γ,IL-4） and Igs（total IgE,β-Lg-specific IgE,total IgG） production by ELISA assay.In vitro studies,4 active/heat-killed lactobacilli could differently modulate the produc-tion of Th1/Th2 cytokine and antibody,especially the heat-killed Lactobacillus fermentum and Lacto-bacillus acidophilus were not inferior to the live strains.These two heat-killed Lactobacillus suppressed IL-4 and IL-10 production by stimulating IL-12 and IFN-γ production.Their IFN-γ/IL-4 value,which represents the Th1/Th2 cell balance were higher than that of other strains,and compared with the con-trol were significant（P0.05）.At the same time,these two heat-killed strains significantly decreased the secretion of total IgE,β-Lg-specific IgE and total IgG（P0.05）.These data indicate that lactobacilli may be effective in decreasing the antibody production in sensitized-mice splenocytes by increasing the ratio of IFN-γ/IL-4 and changing the dominant of Th2.Four strains may differ in their respective ability to improve Th1/Th2 cell balance toward Th1 dominance.

关 键 词：乳杆菌 Β-乳球蛋白 过敏 TH1/TH2细胞平衡 抗体 

分 类 号：Q93[生物学—微生物学]