白假丝酵母脂肪酶CaLIP5在毕赤酵母X33中的优化重组表达  

Optimum Production of Recombinant Candida albicans Lipase 5 Expressed in Pichia pastoris X33

作　　者：杨宁[1] 蓝东明[1] 王永华[2] 杨博[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]华南理工大学食品科学与工程学院,广东广州510641

出　　处：《安徽农业科学》2011年第17期10124-10126,10129,共4页Journal of Anhui Agricultural Sciences

基　　金：广州市科技攻关项目(2009A1-E)

摘　　要：[目的]研究新的脂肪酶及优化脂肪酶发酵培养条件。[方法]在毕赤酵母X33系统中重组表达白假丝酵母脂肪酶CaLIP5。利用响应面分析法,构建了CaLIP5的酶活回归方程。[结果]由响应面分析可知,在温度25.71℃,pH8.33,酵母提取物浓度1.28%和葡萄糖浓度4.06%时,预测最大酶活为8.33U/ml。试验验证酶活为9.24U/ml。实际试验值与模型预测值基本一致。优化后的酶活提高了22.22%。[结论]在优化的培养条件下,可以获得毕赤酵母X33重组表达CaLIP5的最大酶活。[ Objective ] To develop new lipases and optimize the fermentation medium of lipase. [ Method ] The lip5 gene of C. albicans was first cloned and heterogenously expressed in Pichia pastoris X33 system. Response surface methodology was adopted to construct the regression equation for the lipolytic activity of CaLIPS. [ Result] Under 25.71℃, pH 8.33, 1.28% yeast extract, and 4.06% glucose concentration, the predicted maximum lipolytic activity was 8.33 U/ml, and the actual value was 9.24 U/ml. The actual value and the predicted value were basically the same. The optimized lipolytic activity was 22.22% higher than before. [ Conclusion] Highest lipolytic activity of recombinant CaLIP5 was obtained in the ootimized fermentation progress.

关 键 词：白假丝酵母脂肪酶 重组表达 优化培养基 响应面法 

分 类 号：S132[农业科学—农业基础科学]