超声辅助提取白背三七总黄酮  

Ultrasonic-assisted Extraction of Total Flavonoids from Gynura divaricata L. DC

作　　者：李辉[1,2] 卜晓英[3] 陈功锡[1,2] 李亚男[1] 梁春凤[1] 谢依蓁[1] 

机构地区：[1]吉首大学生物资源与环境学院,湖南吉首416000 [2]植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [3]林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《食品科学》2011年第14期144-146,共3页Food Science

基　　金：湖南省教育厅优秀青年项目(06B076)

摘　　要：目的:超声辅助提取白背三七干叶、鲜叶、干茎和鲜茎中的总黄酮,探讨超声促进提取的原因。方法:用单因素试验法考察液料比、提取时间和乙醇体积分数对黄酮提取率的影响,并与常规溶剂提取法进行对比。用扫描电镜研究超声处理后白背三七叶片的结构改变。结果:超声提取最优条件为每克植物材料用30mL 70%乙醇溶液提取15min,重复提取2次;对白背三七的相同部位超声提取时,新鲜材料提取率明显高于干的植物材料。结论:超声提取白背三七总黄酮时,提取效率较高、时间短,其高效率可能来源于超声波对材料表明细胞壁的强烈损伤。Objective： To optimize the ultrasonic-assisted extraction （UAE） of total flavonoids from dried and fresh leaves and stems of Gynura divaricata L. DC and explore the mechanisms by which ultrasonic can enhance the extraction of total flavonoids. Methods： The effects of material-to-liquid ratio, extraction time and ethanol concentration on extraction rate of total flavonoids were explored by single factor experiments. Scanning electron microscope （SEM） was utilized for examining the structural change of Gynura divaricata L. DC leaves after ultrasonic treatment. Results： The optimal UAE conditions were material-to-liquid ratio of 1：30 （g/mL）; extraction time of 15 rain, ethanol concentration of 70%, and number of repeated extractions of 2. Under these conditions, the extraction rate of total flavonoids from the same parts of fresh Gynura divaricata L. DC was higher than that from dried Gynura divaricata L. DC. Conclusion： UAE method has many advantages over conventional solvent extraction such as higher extraction efficiency and shorter extraction time, which is possibly due to severe damage of Gynura divaricata L. DC cell wall by ultrasound.

关 键 词：超声辅助提取 白背三七 总黄酮 

分 类 号：R284.2[医药卫生—中药学] O623.54[医药卫生—中医学]