响应面法优化螺旋藻自溶条件的研究  

Optimizing conditions for autolysis of Spirulina using response surface methodology(RSM)

作　　者：冯强[1] 滕建文[1] 黄丽[1] 韦保耀[1] 

机构地区：[1]广西大学轻工与食品工程学院,南宁530004

出　　处：《食品科技》2011年第7期95-98,102,共5页Food Science and Technology

基　　金：广西科技攻关重点项目(桂科攻0815009-2-4);北海市科技攻关项目(北科合200801026)

摘　　要：以新鲜螺旋藻藻泥为原料,通过响应面实验设计方法对螺旋藻自溶条件进行优化。以可溶性肽含量为响应变量,温度、pH以及Na+浓度为自变量,应用中心复合设计-一致精度(CCD Uniform precision)法设计实验,轴值取1.43,6个中心点,共20组实验。实验结果表明,螺旋藻自溶最适温度为48.6℃、pH为9.6以及Na+浓度为1.00mol/L,最适条件下螺旋藻自溶1hTCA-可溶性肽含量比未优化(室温25℃,加蒸馏水)下高6.35倍。The autolysis of Spirulina was optimised using response surface methodolygy.Twenty test were conducted following a Central Composite Designs-Uniform precision（CCDU） followed by ridge analysis using the TCA-solution peptides concentration,the autolysis temperature,pH and Na＋ concentration as the independent variables and taking the TCA-solution peptides concentration as the response variable.The optimal autolysis conditions were temperature of 48.6 ℃,pH of 9.6 and Na＋ concentration of 1.0 mol/L,which gave a maximal TCA-solution peptides concentration of 12.819 mg Tyr./g Spirulina.Through the optimization of autolysis conditions,the TCA-solution peptides concentration is promoted by 6.35 times.

关 键 词：螺旋藻 自溶 响应面实验 多肽 

分 类 号：TS949.2[轻工技术与工程—服装设计与工程]