董酒中萜烯类物质的研究  

Research on Terpenoids in Dongjiu

作　　者：胡光源[1] 范文来[1] 徐岩[1] 贾翘彦 冉晓鸿 

机构地区：[1]教育部工业生物技术重点实验室江南大学酿酒科学与酶技术研究中心酿造微生物与应用酶学研究室,江苏无锡214122 [2]董酒股份有限公司,贵州遵义563003

出　　处：《酿酒科技》2011年第7期29-33,共5页Liquor-Making Science & Technology

基　　金：黔科合重大专项字[2010]6004号

摘　　要：应用正相色谱技术串联气相色谱-质谱法(GC-MS)检测董酒中萜烯类化合物。通过液液萃取(LLE)分离得到董酒中性/碱性组分。中性/碱性组分过硅胶柱,并分别用戊烷-乙醚混合液(体积比分别为100∶0、95∶5、90∶10、80∶20、70∶30和50∶50)和甲醇洗脱后得到7个组分,应用GC-MS对各个组分进行分析。通过质谱谱库检索、标准品的保留指数(RI)比对以及参考文献的保留指数(RIL)比对分析,共鉴定出52种萜烯类化合物。通过峰面积百分比分析,发现p-茴香醛浓度最高(27.21%),其次是白菖油萜(11.31%),δ-杜松烯(7.38%),卡拉烯(5.11%),樟脑(4.13%),(-)-龙脑(3.65%),α-雪松烯(3.29%)等。这些萜烯类化合物很少在白酒中检测到,推测其来源于制曲过程中添加的中草药。Terpenoids in Dongjiu were detected by normal phase chromatography coupled with gas chromatography-mass spectrometry（GC-MS）.In the experiments,Dongjiu liquor samples were firstly treated by liquid-liquid extraction（pentane：ethyl ether（1∶1））,and then fractionated into water-acidic and neutral/basic fractions.The neutral/basic fraction was further separated into seven fractionations by using silica gel normal phase chromatography,pentane-diethyl ether solution（the volume ratio of pentane and diethyl ether were 100∶0（F1）,95∶5（F2）,90∶10（F3）,80∶20（F4）,70∶30（F5）,and 50∶50（F6） respectively）,and 100 % methanol（F7）.Each fraction was analyzed by GC–MS.A total of 52 terpenoids were finally identified by mass spectrum library search,Kovats retention indices of the standards（RI） or from the literature（RIL）.Peak area percentage analysis results showed that the concentration of p-anisaldehyde was the highest（27.21 %） among all detected terpenes,and then calarene（11.31%）,δ-cadinene（7.38 %）,calamenene（5.11 %）,camphor（4.13 %）,（-）-borneol（3.65 %）,α-cedrene（3.29 %） and so on.These terpenoids were rarely detected in Chinese liquor,and probably came from the herbs added in starter-making.

关 键 词：萜烯类化合物 董酒 正相色谱 气相色谱-质谱 

分 类 号：TS262.3[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]