膜种类及前处理方式对原奶中葡萄球菌肠毒素层析试纸条检测的影响  

The Influence of Membrane's Type and the Pretreatment Methods to the Detection of Staphylococcal Enterotoxin A,B and C in Raw Milk by Lateral Flow Test Strips

作　　者：林松[1] 何艳玲[1] 王丽哲[1] 赵瑜[1] 袁飞[1] 杨海荣[1] 陈颖[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《食品与发酵工业》2011年第5期157-159,165,共4页Food and Fermentation Industries

基　　金：国家科技支撑计划(2009BAD9B06);国家质检总局科技计划项目(2009IK315;2009IK312)

摘　　要：对用于原料奶中葡萄球菌A、B、C型肠毒素现场快速检测的免疫层析试纸条进行了初步研究。研究内容主要包括层析膜和原奶预处理方法的选择及优化,通过实验,最终选择了型号为HF09002的层析膜,采用了2倍稀释的样品预处理方法,研制所得的3种免疫层析试纸条分别检测原奶中SEA的灵敏度为10 ng/mL,SEB的灵敏度为4 ng/mL,SEC的灵敏度为8 ng/mL,与3 MTM TecraTM微孔板法检测结果相比无显著性差异。To establish rapid assays for individual detection of Staphylococal Enterotoxin A, B and C （SEA, SEB, SEC） in raw milk samples, Lateral Flow Immunoassay test strips were studied. The models of nitrocellulosemembrane and the pretreatment methods of raw milk were chosen and optimized, and the membrane of HF09002 and the method of 2 times dilution showed the best result for the field detection of raw milk samples. The Sensitivties of Staphylococcal Enterotoxin A, B and C test strips were 10ng/ mL, 4ng/ mL and 8ng/ mL, and there is no observe difference objected that compared with 3M（tm） Tecra（tm） Staph Enterotoxin ID Test method.

关 键 词：葡萄球菌肠毒素A B C 原料奶 横向侧流免疫检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]