星点设计-效应面法优化双水相-超声提取珍珠草多酚  

Optimization of Ultrasound-assisted Aqueous Two-phase Extraction of Polyphenols from Phyllanthus urinaria L.by Central Composite Design and Response Surface Methodology

作　　者：高云涛[1,2] 蒋进超[1,2] 张宝通[1,2] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650500 [2]国家民委-教育部共建民族药资源化学重点实验室,云南昆明650500

出　　处：《食品与发酵工业》2011年第5期189-193,共5页Food and Fermentation Industries

基　　金：云南省社会发展科技计划项目(2007B148M);云南省教育厅科学基金资助项目(06Y195C);国家民委-教育部共建民族药资源化学重点实验室开放基金项目(MJY07010)

摘　　要：利用超声与丙醇-硫酸铵双水相体系耦合对珍珠草多酚进行提取分离,基于单因素实验得到适宜条件:丙醇体积分数(A)60.0%、料液比(B)为1∶25.0(g∶mL)、超声时间(C)为30.0 min、(NH4)2SO4用量(D)为0.35g/mL。设计了四因素五水平的星点设计-效应面分析对提取条件进行优化,获得的最佳提取条件是:丙醇体积分数53.65%、料液比为1∶23.97(g∶mL)、超声时间为28.19 min、(NH4)2SO4用量为0.40 g/mL,珍珠草总多酚提取率E预测值为8.67%,与实验值E=8.72%(n=5)相符,优于单因素试验优化结果(8.61%,n=5)。Ultrasound-assisted propyl alcohol ammonium sulfate aqueous two-phase extraction of polyphenols from Phyllanthus urinaria L. was investigated. The best condition for the single factor experiment was： propyl-alcohol concentration 60.0% , liquid/solid ratio B 25.0, extraction time C 30min. A Response surface methodology, with a five levels, four variables central composite designs was applied based on the single factor optimization experiment. The best condition was： propyl-alcohol concentration 53.65% , liquid/solid ratio 23.97, extraction time 28. 19 min, and ammonium sulfate concentration 0.40 g/ml. An experimental extraction yield of 8.72 % （n = 5） was achieved under the optimal extraction which was very close to the optimal prediction extraction yield （8.67 % ） ,and was higher than that of single factor optimization experiment（8.61% ,n = 5）.

关 键 词：超声 丙醇-硫酸铵双水相 提取 珍珠草 植物多酚 星点设计 效应面分析 

分 类 号：TQ461[化学工程—制药化工]