松茸不同溶剂提取物及其抗氧化活性  

Study on Antioxidant Activity of Different Polar Solvents Extracts and the Effective Substance of Tricholoma matsutake Sing

作　　者：尹秀莲[1] 游庆红[1] 蒋中海[1] 郑尚永[1] 

机构地区：[1]淮阴工学院生命科学与化学工程学院,江苏淮安223003

出　　处：《食品与发酵工业》2011年第5期194-198,共5页Food and Fermentation Industries

基　　金：江苏省科技计划(BE2009308-4)

摘　　要：对松茸不同溶剂提取物及其抗氧化活性进行了研究。依次用石油醚、三氯甲烷、乙醇及水提取,乙醇提取部分依次用乙酸乙酯和正丁醇萃取。采用DPPH自由基法、邻苯三酚自氧化法,以BHT、Vc为参照,分别研究松茸不同溶剂提取物的抗氧化活性,并对不同溶剂提取物中的总多糖、总黄酮及总多酚含量进行测定。结果表明:松茸不同溶剂提取物均具有一定的抗氧化活性,其中乙酸乙酯、水提取物的抗氧化活性较强,不同溶剂提取物的抗氧化活性与其总多糖、总黄酮和总多酚的含量具有一定的相关性。The antioxidant activity of different polar solvents extracts and the effective substance of Tricholoma rnatsutake were studied. Dried Tricholoma matsutake Sing was sequentially extracted with petroleum ether, chloroform, ethanol and water, the ethanol extract was fractionated into ethyl acetate and n - butyl alcohol - soluble fractions. Using BHT, Vc as reference, the antioxidant activity of the extracts was evaluated by DPPH radical scavenging and pyrogallol auto - oxidation assay, then quantity of the total polysaceharide, total flavonoids, total phenols of dif- ferent fraction were analyzed. Results show that all the extracts exhibited some antioxidant abilities in a dose - dependent manner. The ethyl acetate fraction and the water fraction showed the highest antioxidant activity, and the antioxidant activity has correlation with the content of total polysaecharide, total flavonoids, total phenols.

关 键 词：松茸 提取物 抗氧化活性 

分 类 号：R285[医药卫生—中药学] R284[医药卫生—中医学]