BHRF1反义寡核苷酸对BHRF1-CNE2细胞生存能力的影响  

Effect of BHRF1 antisense oligonucleotide on the vitality of BHRF1 CNE2 cells

作　　者：黄海[1] 周江红[1] 潘星华 余龙[3] 孔宪涛[4] 

机构地区：[1]第二军医大学长海医院药学部 [2]BoyerCenterforMolecularMedicine,YaleUniversity [3]复旦大学遗传学研究所基因工程国家重点实验室 [4]第二军医大学长征医院全军临床免疫中心

出　　处：《第二军医大学学报》1999年第10期720-722,共3页Academic Journal of Second Military Medical University

基　　金：军队"九五"医学科研规划基金

摘　　要：目的：探讨ＢＨＲＦ１ 反义寡核苷酸对表达ＢＨＲＦ１ 的鼻咽癌细胞株ＣＮＥ２ 生存能力的影响。方法：检测反义阻断ＢＨＲＦ１ 表达后ＢＨＲＦ１－ＣＮＥ２细胞在喜树碱作用下增殖能力和凋亡指数的变化。结果：在喜树碱作用下，应用ＢＨＲＦ１ 反义寡核苷酸组的癌细胞较对照组的增殖率下降，凋亡率增高。结论：反义阻断ＢＨＲＦ１的表达可降低ＣＮＥ２细胞的生存能力。Objective: To investigate the effect of BHRF1 antisense oligonucleotide on apoptotic resistance of the BHRF1 expressing cell line of nasopharyngeal carcimona, CNE2. Methods: After incubated with BHRF1 antisense oligonucleotide, BHRF1 CNE2 cells were treated with camptothecin, and tested for the alterations of proliferative ability and apoptotic index. Results: After the treatment of camptothecin, in antisense group, proliferative rates of CNE2 dropped and apoptotic rates increased. Conclusion: Antisense inhibition of BHRF1 expression can reduce apoptotic resistance of CNE 2 cells, which may be valuable for the treatment of nasopharyngeal carcinoma.

关 键 词：鼻咽肿瘤 BHRF1基因 反义寡核苷酸 CNE2细胞 

分 类 号：R739.63[医药卫生—肿瘤]