Ru(bpy)_3^(2+)体系电化学发光法测定阿奇霉素的研究  

Electrochemiluminescencial Study on the Determination of Azithromycin using Ru(bpy)_3^(2+)System

作　　者：李利军[1] 邓春燕[1] 高文燕[2] 胡大春[1] 李彦青[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]广西大学化学和化学工程学院,南宁530004

出　　处：《化学通报》2011年第7期646-650,共5页Chemistry

基　　金：国家自然科学基金项目(20665001);广西科学基金项目(0832062)资助

摘　　要：实验发现,阿奇霉素对联吡啶钌的电致化学发光(ECL)具有显著的增强作用。据此,建立了以金电极为工作电极的测定阿奇霉素ECL分析新方法。采用了循环伏安(CV)和ECL法,研究了阿奇霉素对联吡啶钌体系的电化学行为和ECL行为的增强作用。结果表明,在最佳条件下,阿奇霉素浓度在2.0×10-4～4.0×10-7 mol/L范围内与相对发光强度呈线性关系,其线性回归方程为I计数=22.848×106 C+221.8(r=0.9981,n=12)。检出限为3.00×10-9 mol/L(S/N=3)。连续平行测定1.0×10-5 mol/L的阿奇霉素标品溶液10次,发光强度值的相对标准偏差(RSD)为2.09%。对样品进行回收率试验,回收率在95.0%～102.0%之间,RSD为2.40%(n=5)。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于药物中阿奇霉素的测定,结果满意。The addition of Azithromycin would further lead to an enhancement of ECL intensity of Ru（bpy）3^2＋.Based on this,a new ECL method was established for the determination of Azithromycin at gold electrode,and the electrochemical and ECL behaviors of this system were studied by cyclic voltammetry and electrogenerated chemiluminescence.The results showed that the ECL peak counts increased linearly with the concentration of Azithromycin in the range of 2.0×10^-4～4.0×10^-7mol/L under the optimal conditions,linear regression equation was Icounts=22.848×10^6C＋221.8（r=0.9981,n=12）.The detection limit for Azithromycin was 3.002×10^-9mol/L（S/N=3）.The RSD for 10 times determination of 1.0×10^-5 mol/L Azithromycin was 2.09%.The results of recovery test were between 95.0%-102.0%,and the RSD is 2.40%.The presented method had the advantage of high selectivity and sensitivity,and the disposal procession of the sample was simple.This proposed method was applied in the determination of Azithromycin capsule with satisfactory results.

关 键 词：阿奇霉素 电致化学发光 联吡啶钌 金电极 

分 类 号：R914[医药卫生—药物化学]