不同抗原修复方法对Ki-67蛋白检测结果的影响  

Effect of different antigen retrieval methods on Ki-67 immunohistochemical staining

作　　者：邹金[1] 李亨芬[1] 邢咏梅[1] 聂金梅[1] 白如玉[1] 刁勇[1] 

机构地区：[1]华侨大学分子药物学研究所,福建省泉州市362021

出　　处：《中华实用诊断与治疗杂志》2011年第7期664-666,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金项目(30973591);国家高技术研究发展计划(863计划)资助(2008AA02Z135);国家科技重大专项课题(2009ZX09103-643);福建省科技项目(BC2009I0017)

摘　　要：目的:探讨不同抗原修复对A549肿瘤组织内Ki-67蛋白检测结果的影响。方法:采用酶修复法、柠檬酸微波修复法、柠檬酸高压修复法、Tris/EDTA微波修复法、Tris/EDTA高压修复法等对肿瘤组织的石蜡切片进行修复,采用二步法进行免疫组织化学染色。结果:肿瘤组织的核蛋白Ki-67经柠檬酸微波修复后,染色效果较其他修复方法明显增强,且定位佳,对比度清晰。结论:在核蛋白Ki-67免疫组织化学染色中,柠檬酸微波修复法优于其他抗原修复方法。Objective To explore the effect of different methods of antigen retrieval on the result of immunohistochemical staining of Ki-67.Methods Antigens were retrieved respectively by using trypsin,citric microwave,citric autoclave,Tris/EDTA microwave and Tris/EDTA autoclave in tumor paraffin sections from nude mouse,followed by immunohistochemical staining.Results After antigen retrieval with citric microwave,the positive staining intensity of nuclear protein Ki-67 was enhanced,and Ki-67 was better located by using of this method than the others.Conclusion In the immunohistochemical staining methods of Ki-67,citric microwave is the best one to retrieve antigen.

关 键 词：免疫组织化学 KI-67 抗原修复 

分 类 号：R361.2[医药卫生—病理学]