炼油厂周边PAHs污染土壤中微生物群落结构多样性研究  

Application of T-RFLP to study microbial diversity in PAHs polluted soil around oil refinery

作　　者：彭静静[1] 张又弛[1] 侯艳伟[2] 黎慧娟[1] 蔡超[1] 

机构地区：[1]中国科学院城市环境研究所//中国科学院城市环境与健康重点实验室,福建厦门361021 [2]华侨大学化工学院环境科学与工程系,福建厦门361021

出　　处：《生态环境学报》2011年第5期962-965,共4页Ecology and Environmental Sciences

基　　金：国家自然科学基金项目(40801118)

摘　　要：直接从污染土壤中提取微生物基因组DNA,对基因组DNA进行16S rDNA聚合酶链式反应(PCR)扩增和末端限制性片段多态性(T-RFLP)分析,进而对炼油厂附近PAHs污染区土壤微生物群落结构和多样性进行初步研究。结果表明,PAHs浓度较高的土壤中PAHs主要以高相对分子质量PAHs为主,此外,其土壤微生物群落多样性明显低于PAHs浓度较低的土壤。高浓度PAHs刺激了某些土壤微生物生长。不同污染程度的土壤存在一定数量相同的优势菌群,但相对丰度具有明显差异。其中α-变形菌是五个区域土壤中的主导微生物。研究结果将为炼油厂周边土壤的修复治理提供科学依据。After DNA extraction from oil refinery adjacent soil directly,composition of bacterial communities was evaluated using polymerase chain reaction（PCR）-terminal restriction fragment length polymorphism（T-RFLP） analysis.Bacterial 16S rRNA T-RFLP fingerprinting revealed a high structural bacterial diversity in soil.High molecular weight PAHs is the dominate group in the high PAHs polluted soil.High concentration of PAHs in the polluted soil showed a strong effect on the bacterial populations.Our results revealed that higher bacterial diversity was found in the soil with low levels of PAHs.This indicates that PAHs affect the bac-terial community by favoring certain groups of bacterial community.There were several bacteria detected in the different soils,while the relative abundance of T-RFs differed significantly with different soils.α-Proteobacteria is the dominant group in different soils.This study will provide scientific evidence for bioremediation of soil around oil refinery.

关 键 词：土壤 PCR-T-RFLP 微生物多样性 多环芳烃 

分 类 号：X176[环境科学与工程—环境科学]