转人α-乳清白蛋白基因奶粉亚慢性毒性试验  

Study on sub-chronic toxicity of powered milk containing transgenic human α-lactalbumin

作　　者：支媛[1] 刘海波[1] 耿桂英[1] 汪会玲[1] 杨华[1] 冯晓莲[1] 高芃[1] 余强[1] 冯永全[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《卫生研究》2011年第4期426-430,共5页Journal of Hygiene Research

基　　金：国家转基因生物新品种培育重大专项(No.2008zx08007-001;2008zx08011-005)

摘　　要：目的研究转人α-乳清白蛋白基因奶粉对Wistar大鼠的亚慢性毒性。方法取初断乳Wistar大鼠140只,雌雄各半,按照体重随机分为7组。分别喂饲含有转人α-乳清白蛋白比例为15%、30%和60%成分奶粉的配制饲料,添加比例为15%、30%和60%普通奶粉的配制饲料和基础饲料,喂饲90天。在实验中期(第45天)和实验结束时(第90天)采血检测血液学和血液生化指标。实验结束时处死实验动物,测定脏器绝对重量,计算脏器指数,并对主要脏器进行病理组织学观察。结果转人α-乳清白蛋白基因奶粉组与普通奶粉组和基础饲料组比较,未发现转人α-乳清白蛋白基因奶粉对动物体重、食物利用率、血液学、血液生化、脏器系数和病理组织学观察有生物学意义的改变。结论给予大鼠转人α-乳清白蛋白基因奶粉90天未发现该奶粉对实验动物有毒性作用。Objective To investigate the potential toxic or adverse effect of transgenic human α-lactalbumin powered milk on rats.Methods Weanling Wistar rats were randomly divided into seven groups according the weight： three transgenic milk powder（T） groups,three non-transgenic milk powder（N） groups and the control（C） group.The diets of T groups contain 15%,30% and 60% transgenic human α-lactalbumin milk powder.The diets of N groups contain 15%,30%and 60% non-transgenic human α-lactalbumin milk powder for 90 days.The diet of C group contains only basic feed.Haematological and biochemical parameters was measured during the study（at 45th and 90th of the experiment）.At the end of the 90th day,organ tissues analysis was performed.Results There were no transgenic human α-lactalbumin related adverse effects on the body weight,food intake,food consumption,hematology,serum biochemistry,as well as histopathology.Conclusion There were no signs of toxic and adverse effects for transgenic human α-lactalbumin powdered milk on rats.

关 键 词：转基因奶粉 α-乳清白蛋白 亚慢性毒性 

分 类 号：R155.51[医药卫生—营养与食品卫生学] TS201.6[医药卫生—公共卫生与预防医学]