同时高产甘露聚糖肽与乳酶生发酵条件的研究  

Research for the Fermentation Condition of High Yield Mannatide and Lactasin Simultaneously

作　　者：冯锁民[1] 任淑娟[2] 杨颖[1] 王黎[1] 惠乐妮 

机构地区：[1]西安医学院,陕西西安710021 [2]陕西省人民医院药剂科,陕西西安710068 [3]陕西康惠制药股份有限公司,陕西咸阳712000

出　　处：《安徽农业科学》2011年第19期11889-11891,共3页Journal of Anhui Agricultural Sciences

基　　金：2008年西安医学院博士科研启动金项目(2008DOC3);2010年陕西省卫生厅科研基金项目(2010D03)

摘　　要：[目的]探索同时高产甘露聚糖肽和乳酶生的最佳发酵条件。[方法]以甲型溶血性链球菌H1S-33号为菌株,进行液体发酵培养,在各个时间段分别取样测定活菌数及甘露聚糖肽含量,确定最佳培养条件。[结果]在液体发酵过程中,28h发酵液中甘露聚糖肽含量最高为69.2μg/ml,活菌数最多为2.38×1016个/ml。[结论]同时高产甘露聚糖肽与乳酶生发酵条件为:培养基由牛肉膏、蛋白胨、酵母膏、氯化钠组成,发酵温度37℃,最佳发酵时间28h。[Objective]The aim was to explore the fermentation condition of high yield mannatide and lactasin simultaneously.[Method]In order to obtain the best culture conditions,in the period of liquid fermentation culture,using α-hemalytic streptococci 33 as strains,all living bacteria number and mannatide content were determined respectively.[Result]In the liquid fermentation process,the highest content of mannatide was 69.2 μg/ml,the maximum number of living bacteria was 2.38 × 1016 /ml.[Conclusion] The best fermentation conditions were made from beef cream medium,peptone,yeast creams,sodium chloride composition,at temperature 37 ℃ with 28 h.

关 键 词：甘露聚糖肽 乳酶生 甲型溶血性链球菌 发酵 培养基 细菌计数 

分 类 号：S37[农业科学—农产品加工]