CCR7-SLC/ELC生物学轴对乳腺癌细胞趋化及侵袭活性的影响  

CC chemokine receptor-7 and its ligand SLC,ELCS influence on chemotaxis and inyvasion of breast cancer cells

作　　者：朱丽华[1] 谢明均[1] 

机构地区：[1]宜宾市第一人民医院乳腺外科,四川宜宾644000

出　　处：《四川医学》2011年第7期988-991,共4页Sichuan Medical Journal

基　　金：四川省科技厅资助项目(编号:2008FG0021)

摘　　要：目的探讨CCR7-SLC/ELC生物学轴对乳腺癌细胞株MCF-7的趋化及侵袭活性的影响。方法用Boyden趋化小室测定外源性趋化因子SLC、ELC对MCF-7趋化及侵袭活性的影响。用Westen-Blot法检测所取新鲜乳腺癌转移淋巴结标本中SLC、ELC的表达情况。取转移淋巴结组织匀浆上清液代替外源趋化因子,测定其对MCF-7趋化及侵袭活性。同时测定使用SLC、ELC单克隆抗体后趋化及侵袭活性的改变。结果不论是外源性SLC、ELC还是转移淋巴结的蛋白上清液均能增加MCF-7趋化及侵袭活性。SLC、ELC单克隆抗体的封闭能明显抑制其活性。结论 CCR7-SLC/ELC生物学轴增加乳腺癌细胞的趋化和侵袭活性,可能参与乳腺癌的亲嗜性淋巴结转移。Objective To study the chemotaxis and invasion of breast cancer cells-MCF-7 facilitated by CC chemokine receptor-7（CCR7） and its ligand second lymphoid tissue chemokine（SLC） EBII-ligand chemokine（ELC）.Methods The chemotaxis and invasion assays were performed on breast cancer cells-MCF-7 in presence of exogenous SLC and ELC.First detect the expression of SLC and ELC in novel lymph node with breast cancer metastasis by Westen-Blot.Second replace the exogenous SLC and ELC with the clear supernatant liquid of novel lymph node with breast cancer metastasis to study the chemotaxis and invasion of breast cancer cells-MCF-7 facilitated by endogenous SLC and ELC and when SLC and ELC was blocked.Results Both exotgenous and endogenous SLC and ELC can facilitate the chemotaxis and invasion of the breast cancer cells-MCF-7,both chemotaxis and invasion could be blocked by neutralazing anti-SLC,ELC antibody.Conclusion Chemotaxis and invasion of breast cance cell can be facilitated by axis of CCR7/SLC,ELC and the axis may play an important role in the lymph node metastasis of breast cancer.

关 键 词：CCR7 SLC ELC 乳腺癌细胞 趋化活性 侵袭活性 

分 类 号：R737.9[医药卫生—肿瘤]