富硒酵母对斑点叉尾肝脏的毒性作用及蛋白质组学的影响  

Proteomic analysis on hepatotoxicity of selenium-enriched yeast to channel catfish(Ictalurus punctatus) liver

作　　者：熊华伟[1] 简少卿[1] 贾小芳[2] 虞舜[1] 何宝荣[1] 曾艳[1] 张丽军[2] 虞鹏程[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,江西南昌330031 [2]上海市(复旦大学附属)公共卫生临床中心,上海201508

出　　处：《水产学报》2011年第7期1008-1014,共7页Journal of Fisheries of China

基　　金：江西省科技厅重大招标项目(20051A0600400);上海市自然科学基金(09ZR1426300)

摘　　要：实验探讨了富硒酵母对斑点叉尾肝脏的毒性作用及蛋白质组学的影响,结果表明,饲养56 d后正常组和有机硒组采用原子荧光法检测硒含量均值为0.15和0.81 mg/kg。H.E染色显示有机硒组肝细胞有明显的疏松化,部分发生脂肪变。通过二维凝胶电泳结合ImageMaster软件分析,鉴定出8个表达差异蛋白质点,其中3个在有机硒组表达上调,5个在有机硒组特异表达,经液相色谱串联质谱分析鉴定,这些蛋白质分别是伴侣蛋白TCP1-亚基8、甘油醛-3-磷酸脱氢酶、4SNc-Tudor蛋白、腺苷激酶、酮糖移转酶、丙氨酰-tRNA合成酶。富硒酵母饲料对斑点叉尾肝具有明显的毒性效应,有机硒调控4SNc-Tudor蛋白,通过信号传导通道加强机体免疫。To investigate the influence on channel catfish（Ictalurus punctatus）liver toxicity and liver proteome change by the use of selenium-enriched yeast,Se-concentration in channel catfish liver was determined and the pathological change was monitored after selenium-enriched yeast feeding for 56 days.Proteins extracted from the liver of selenium-enriched yeast feeding channel catfishes and non-feeding controls were separated by two-dimensional gel electrophoresis（2-DE）,differentially expressed proteins between the two groups were analyzed using ImageMaster software and identified by online reversed-phase nano-flow liquid chromatography coupled with ion trap mass spectrometry（LC-MS/MS）.The average Se-concentration of liver form the non-feeding controls and the selenium-enriched yeast feeding ones were 0.15 mg/kg and 0.81 mg/kg.Significant difference was detected between these two groups（P0.05）.H.E staining of pathology from channel catfish liver showed that the selenium-enriched yeast group has obvious cell loose and cell fatty degeneration.15 differetial protein were found by 2-DE separation and ImageMaster software analysis.Of them,8 over-expressed proteins in selenium-enriched yeast were selected for MS analysis and identified successfully,including 3 u-regulated proteins and 5 uniquely expressed proteins in selenium-enriched yeast group compared with the controls.These differential proteins correspond to chaperonin TCP1 subunit 8 and glyceraldehyde-3-phosphate dehydrogenase,4SNc-Tudor domain protein,adenosine kinase a,transketolase and alanyl-tRNA synthetase-like et al.Obvious toxicity was detected in the channel catfish liver after feeding with selenium-enriched yeast.Organic selenium might strengthen immune through 4SNc-Tudor protein involved signal transmission pathway.Differential proteins identified in our work could be specific biomarkers related to toxicity of organic Se to channel catfish.

关 键 词：斑点叉尾鮰 富硒酵母 双向电泳 蛋白质组学 

分 类 号：S917[农业科学—水产科学]