GST对转染APP695基因PC12细胞形态和增殖能力的影响  

Effect of Genistein against apoptosis and proliferation of PC12 cells transfected with mutant APP695 type

作　　者：赵翠香[1] 张丽娟 龙云[3] 王凤斌[1] 

机构地区：[1]潍坊医学院生理学教研室,潍坊261053 [2]潍坊市坊子区人民医院脑科医院,潍坊261053 [3]重庆三峡医药高等专科学校,重庆400000

出　　处：《神经解剖学杂志》2011年第4期399-403,共5页Chinese Journal of Neuroanatomy

基　　金：山东省自然科学基金(Y2004C29);山东省科技发展计划项目(2007GG30002007)

摘　　要：目的:观察APP695MT基因转染对PC12细胞形态和增殖能力的影响及植物雌激素三羟基异黄酮(Genistein,GST)的干预作用,初步探讨GST对表达APP695MT基因PC12细胞的保护作用。方法:以pIRES2-EGFP空载体和pIRES2-EGFP/APP695MT表达载体转染正常PC12细胞,电镜观察各组细胞形态学改变,细胞计数方法检测各组细胞增殖能力差异。结果:电镜下观察可见正常对照组与空载组细胞形态无差异,细胞形态结构正常,无明显病理改变;APP695转染组细胞胞体肿胀,线粒体髓样变和空泡样变,胞核扭曲凹陷,异染色质边集;GST干预组较APP695转染组细胞形态明显改善,线粒体髓样变和空泡样变明显减少,胞核凹陷明显减轻。细胞计数结果显示,在各细胞计数时间点,与正常对照组比较,空载组细胞数目无显著变化(P>0.05),APP695转染组PC12细胞数目减少(P(0.01),细胞生长增殖能力明显受到抑制;GST各剂量处理组与APP695转染组比较细胞生长增殖能力均有不同程度的增强(P(0.01)。结论:GST能改善突变型APP695基因转染引起的PC12细胞病理变化,并提高其增殖能力,对转染细胞有一定的保护作用。Objective： To investigate the protective effect of Genistein against the apoptosis and proliferation of PC12 cells induced by transfection mutant APP695 plasmid DNA.Methods： PC12 cells were transfected with pIRES2-EGFP plasmid DNA and pIRES2-EGFP/APP695MT plasmid DNA,The apoptosis of cells were detected by electron microscope and the proliferation of cells were detected by cell counting.Results： Compared with the normal control group,there were no significant difference between the unladen group and the control groups at every measurement points,cells were normal and had no obviously pathological changes.But at every measurement points,the cells of the APP695 transfected groups were slightly big,the amount of microvilli were more than that in normal control group,mitochondrial had medullary and vacuolation change,nuclear distorted and depressed and heterochromatin marginalized.Compared with the APP695 transfected group,cells of GST-intervention groups at every measurement points were significantly improved and regenerated,the phenomenon of medullary and vacuolation change of mitochondrial and depression of nucleus was significantly reduced.The result of cell counting ： compared with the normal control group,there were no significant difference in the change of cell growth and proliferation in the unladen group（P〉0.05）;cells’ growth and proliferation of the APP695 transfected group were significantly inhibited（P〈0.05）;Compared with the APP695 transfected group,cells’ proliferation of the two GST treatment groups was improved to some extent（P〈0.05）.Conclusion： GST can decrease the apoptosis of PC12 cells induced by transfected with mutant APP695 plasmid DNA and enhance cells’ proliferation,it may also has protective effects on neurons.

关 键 词：GENISTEIN APP695 PC12细胞 细胞形态 增殖能力 

分 类 号：R749.16[医药卫生—神经病学与精神病学]