咖啡因对乙醛刺激的大鼠肝星状细胞HSC-T6增殖、凋亡的影响及部分作用机制  

Effect of caffeine on proliferation,apoptosis in hepatic stellate cell-T6 stimulated by acetaldehyde and its partial mechanisms

作　　者：代雪飞[1] 吕雄文[1] 管文婕[1] 杨万枝[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院,合肥230032

出　　处：《安徽医科大学学报》2011年第8期772-775,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:11040606M194);安徽省高等学校省级自然科学研究重点项目(编号:KJ2008A048)

摘　　要：目的探讨咖啡因(CAF)对乙醛刺激的大鼠肝星状细胞(HSC)-T6增殖、凋亡的影响及部分作用机制。方法用不同浓度的CAF(0.5、1、2、4、8 mmol/L)对乙醛刺激的HSC-T6进行处理,四甲基偶氮唑蓝(MTT)法检测细胞增殖;流式细胞仪检测细胞凋亡及细胞周期分布;RT-PCR法检测HSC-T6中平滑肌肌动蛋白(α-SMA)、肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体DR4、DR5的mRNA表达。结果 CAF对乙醛刺激的大鼠HSC-T6增殖具有抑制作用,阻滞细胞于G0/G1期;能够明显下调HSC-T6中α-SMA的mRNA表达,上调DR4、DR5的mRNA表达。结论 CAF对乙醛刺激的HSC-T6增殖具有抑制作用,并能够促进其凋亡,其机制可能与TRAIL受体DR4、DR5的表达有关。Objective To explore the effect of caffeine on proliferation,apoptosis in hepatic stellate cell-T6（HSC-T6） stimulated by acetaldehyde and its partial mechanisms.Methods HSC-T6 stimulated by acetaldehyde was incubated with different doses of caffeine（0.5,1,2,4,8 mmol/L）,cell proliferation was analyzed by MTT colorimetric assay,apoptosis rate and the cell cycle distribution were analyzed by flow cytometry（FCM）.The mRNA expressions of α-SMA,TRAIL receptor DR4 and DR5 were measured by RT-PCR.Results Caffeine could inhibit HSC-T6 stimulated by acetaldehyde,the cells were accumulated in the G0/G1 phase.The mRNA expression levels of α-SMA decreased,but DR4 and DR5 increased.Conclusion Caffeine can inhibit the growth of HSC-T6 stimulated by acetaldehyde and promote the apoptosis,the mechanisms may be related to the expression of TRAIL receptor DR4 and DR5.

关 键 词：咖啡因 乙醛 细胞凋亡 

分 类 号：R971.7[医药卫生—药品] R916.4[医药卫生—药学]