二甲基亚砜法提取海洋红酵母β-胡萝卜素的最佳工艺  

The Optimum Extraction Technology of β-carotene from Rhodotorula benthica by DMSO Method

作　　者：周鲜娇[1] 吴雪梅[1] 

机构地区：[1]湛江师范学院生命科学与技术学院,广东湛江524048

出　　处：《贵州农业科学》2011年第7期184-187,共4页Guizhou Agricultural Sciences

基　　金：湛江师范学院自然科学研究项目"酵母菌处理水产养殖水质的应用研究"(QL0604);湛江市科技攻关计划"海洋红酵母色素的研究"[湛科(2009)64]

摘　　要：为了进一步探明二甲基亚砜法(DMSO法)提取海洋红酵母中β-胡萝卜素的最佳工艺,进而为海洋红酵母中β-胡萝卜素的工业化生产提供可靠的理论依据,采用DMSO法研究了温度、时间和料液比等因素对酵母菌株HN-3中β-胡萝卜素提取的影响。结果表明,用DMSO法提取海洋红酵母中β-胡萝卜素的最佳条件为:料液比1∶25,提取时间60 min,提取温度30℃。在此条件下提取的色素量高达345.7μg/g,比单因素条件下提取的(307.9μg/g)高10.93%。试验建立了海洋红酵母β-胡萝卜素提取的最佳工艺,为海洋红酵母β-胡萝卜素的高效提取奠定了基础。Effects of temperature,time and solid-liquid ratio on the extraction of β-carotene from Rhodotorula benthica（HN-3） were studied by DMSO method to find out the optimum extraction technology and provide a reliable theoretical basis for industrial production of β-carotene from Rhodotorula benthica.The results showed that the optimum conditions included solid-liquid ratio 1∶25,extraction time 60 min and extraction temperature 30℃,the amount of β-carotene could be up to 345.7μg/g,10.93% higher than that extracted under single factor condition（307.9μg/g）.The optmium extraction technology laid the foundation for efficient extraction of β-carotene from Rhodotorula benthica.

关 键 词：海洋红酵母 Β-胡萝卜素 二甲基亚砜法 

分 类 号：S917.1[农业科学—水产科学]