酸枣仁黄酮组分提取纯化工艺的研究  

Study on Extraction and Purification Technology of Flavones from Zizyphi Spinosi Semen

作　　者：丁轲[1] 谷禹[1] 陆晶晶 伍军[1] 

机构地区：[1]北京农学院食品科学学院,北京102206 [2]中国疾病预防与控制中心营养与食品安全所,北京100021

出　　处：《中国食品学报》2011年第4期62-70,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市优秀人才培养资助项目(No.20081D0502100229);农产品加工及贮藏工程北京市重点建设学科(No.PXM2009_014207_078172)

摘　　要：为了确定从酸枣仁中提取、纯化黄酮组分的最适方法和最佳工艺条件,采用正交试验分别优化回流法和超声法提取黄酮的工艺条件,并筛选适合纯化酸枣仁黄酮组分的树脂,确定上柱试验的最佳工艺参数。试验结果表明:超声法较回流法的提取效果好,其最佳提取条件是:70%乙醇,液固比20∶1,温度40℃,超声功率500W,提取时间2.0h。SP825树脂具有最大的吸附容量和解吸得率,将其装柱后做上柱试验,用35%、45%和55%的乙醇进行阶段式梯度洗脱,结果3种黄酮组分被完全洗脱出来。To obtain the best appropriate method and optimum conditions of extraction and purification of three flavones from Zizyphi spinosi semen（ZSS） ,the conditions of reflux and circulation ultrasonic extraction were optimized by orthogonal design respectively. Mitsubishi SP825 resin was selected from 7 different types of macroporous adsorption resins and the elution conditions of chromatography experiment were optimized. Experimental results showed that circulation ultrasonic extraction was better method than reflux extraction,and its optimized extraction conditions were as follows： 70% ethanol solution,ratio of liquid to solid 20∶1（mL/g） ,ultrasonic power 500 W,2.0 h,40 ℃. SP825 resin was tested with the highest adsorption capacity and yield. In chromatographic experiment using column packed with SP825 resin,Flavones A,spinosin and 6′′′-feruloylspinosin could be eluted entirely with gradient elution mode of 35%,45% and 55% ethanol solution.

关 键 词：酸枣仁 黄酮 超声提取 大孔树脂 

分 类 号：TQ461[化学工程—制药化工]