青霉素G人工抗原的合成及其酶联免疫检测方法研究  

Synthesis of Artificial Antigen of Penicillin G and its Determination by Enzyme-linked Immunosorbent Assay (ELISA)

作　　者：王硕[1] 江月明[1] 宋嘉嘉[1] 于姣[1] 杨泽林[1] 张燕[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院食品营养与安全省部共建教育部重点实验室,天津300457

出　　处：《中国食品学报》2011年第4期168-171,共4页Journal of Chinese Institute Of Food Science and Technology

基　　金：天津市应用基础及前沿技术研究计划重点项目(09JCZDJC19400)

摘　　要：在碱性条件下,降解青霉素G并与6-氨基己酸反应,首次成功合成了纯度较高的半抗原。采用活化酯法与匙孔血蓝蛋白(KLH)偶联,成功制备了具有青霉素G结构特征的人工抗原。通过免疫获得了高效价、高特异性的多克隆抗体,抗血清效价达1∶240000。绘制了青霉素G酶联免疫直接竞争法的标准曲线,检测限可达0.04μg/L,灵敏度0.37μg/L。Penicillin G was degraded in alkaline solution to react with 6-aminocaproic acid（EACA） . High purity of BPO-EACA was firstly prepared. Artificial antigen was produced by coupling the hapten to KLH with an active ester method. The anti-Penicillin polyclonal antiserum with high affinity and specificity was obtained which had a titer of 1∶ 240 000. The stand curve of the direct competitive ELISA for penicillin G was developed. The detection limit and sensitivity were 0.04 μg/L and 0.37 μg/L separately.

关 键 词：青霉素G 人工抗原 酶联免疫 

分 类 号：S859.84[农业科学—临床兽医学]