Using sensitive surface plasmon resonance to detect binding of peptide molecules and immobilized vancomycin  

作　　者：何永辉 刘斌 詹艳峰 刘又年 向娟 

机构地区：[1]School of Chemistry and Chemical Engineering,Central South University

出　　处：《Journal of Central South University》2011年第4期1024-1028,共5页中南大学学报（英文版）

基　　金：Projects(20773165,20876179) supported by the National Natural Science Foundation of China;Project(09JJ1002) supported by the Hunan Provincial Natural Science Foundation,China;Project(NCET-07-0865) for New Century Excellent Talents in Chinese University;Project(2007AA022006) supported by the National High Technology Research and Development Program of China

摘　　要：To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer, the binding between vancomycin and pentapeptide （Lys-Lys-Gly-D-Ala-D-Ala） was investigated by flow injection surface plasmon resonance （FI-SPR） and flow injection quartz crystal microbalance （FI-QCM）. To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation, vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer. By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip, the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved （reaching 0.5 ×10^- 6 mol/L or 7.5 × 10^-12 mol）. The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained （KAds=5.0×10^4 L/tool）.To perform the mechanism study of special association for vancomycin and D-Ala-D-Ala-containing peptides on the interface of solution and self-assemble monolayer,the binding between vancomycin and pentapeptide(Lys-Lys-Gly-D-Ala-D-Ala) was investigated by flow injection surface plasmon resonance(FI-SPR) and flow injection quartz crystal microbalance(FI-QCM).To facilitate the formation of a compact vancomycin adsorbates layer with a uniform surface orientation,vancomycin molecules were attached onto a preformed alkanethiol self-assembled monolayer.By optimizing the conditions for the binding between Lys-Lys-Gly-D-Ala-D-Ala and vancomycin on the assembled chip,the detecting limit of Lys-Lys-Gly-D-Ala-D-Ala was greatly improved(reaching 0.5×10-6 mol/L or 7.5×10-12 mol).The equilibrium constant of the association of Lys-Lys-Gly-D-Ala-D-Ala with vancomycin was also obtained(KAds=5.0×104 L/mol).

关 键 词：flow injection surface plasmon resonance VANCOMYCIN Lys-Lys-Gly-D-Ala-D-Ala BINDING sensitivity 

分 类 号：O53[理学—等离子体物理]