多肽抗体免疫富集-质谱法检测肝癌患者血清多肽标志物  

Determination of Serum Peptide Biomarkers in Primary Hepatocellular Carcinoma by Immunocapture Using Peptide Antibodies and Mass Spectrometry

作　　者：原剑[1,2] 孙云波[1,2] 周晓明[1,2] 杨帆[1,2] 杨保安 郑俊杰[1,2] 甄蓓[1,2] 貌盼勇[4] 李楠[5] 白洁[6] 林利忠 徐淑芳 肖汉族 魏开华[1,2] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]北京蛋白质组研究中心,蛋白质组学国家重点实验室,北京102206 [3]北京正旦国际科技有限责任公司,北京102206 [4]解放军302医院,北京100039 [5]解放军309医院,北京100091 [6]解放军301医院,北京100853 [7]湖南金健药业有限责任公司,常德415001

出　　处：《分析化学》2011年第8期1129-1133,共5页Chinese Journal of Analytical Chemistry

基　　金：卫生部传染病重大专项(No.2008ZX10002-016)资助

摘　　要：血清多肽是癌症诊断信息的重要来源。近年来针对其复杂多样、丰度低、易降解的特点,多种多肽组技术得到快速发展。建立并优化了检测多肽标志物的免疫质谱方法,用于检测血清中的肿瘤多肽标志物。方法的线性范围为1～40pmol;检出限为0.5pmol;对5pmol合成肽5标准品平行检测3次的相对标准偏差(RSD)为9.5%。采用本方法检测出浓度为35.2nmol/L的血清多肽标志物。本方法适用于临床样本中低浓度标志物的检测研究,对于癌症的早期诊断具有重要意义。Serum peptides are the rich sources for cancer-specific diagnostic information and because of their complexity,low abundance,degradation,a lot of new peptidomic technologies were developed in recent years.A method based on peptide antibodies and immuno-mass spectrometry for detection of the serum peptide biomarkers was developed for detecting peptide biomarker candidates in the clinic tumor sera.The linear range of the method was 1-40 pmol with a detection limit of 0.5 pmol.The relative standard deviation was 9.5% for 5 pmol standard of synthetic peptide 5（n=3）.Furthermore,this method was successfully applied to determine the serum peptide biaomarker of 35.2 pmol/mL.The method of immuno-mass spectrometry combining peptide antibodies was paticular suitable for the detection of low concentration biomarkers in clinical samples,and it will be significant for earlier diagnosis of cancers.

关 键 词：多肽抗体 免疫富集 质谱 肿瘤 早期诊断 

分 类 号：R735.7[医药卫生—肿瘤]