HPLC分离黄曲霉毒素生防微生物的拮抗物及拮抗效果检测  

Purify the antagonists of biological-control agent against aflatoxin by HPLC and detect their inhibitive effect

作　　者：王凯[1,2] 闫培生[1] 王琢[2] 任秀莲[1] 郇征宇[1] 

机构地区：[1]哈尔滨工业大学(威海)海洋学院,威海264209 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《食品科技》2011年第8期32-34,共3页Food Science and Technology

基　　金：国家自然科学基金项目(30571244;30870003);哈尔滨工业大学科研创新基金项目(IMXK57080022)

摘　　要：黄曲霉毒素在世界范围内不仅带来巨大的经济损失,而且严重威胁人们的身体健康。生物防治技术在黄曲霉毒素防治方面具有一定优势。利用课题组从土壤中筛选出的一株黄曲霉毒素生防细菌B05,使用HPLC对其代谢产物进行初步分离,确定了分离条件。将分离物收集后通过微孔板培养,确定了各分离组分对黄曲霉毒素的拮抗效果。In the world aflatoxins lead to great economy loss and seriously endanger human health.The biological-control method has advantage on inhibiting aflatoxins.B05 isolated from soil was used as biological-control agent against aflatoxins.The antagonists of biological-control agent was purified by using HPLC and the purification condition was confirmed.The isolates were collected and their inhibitive effect was detected by microtiter agar plate assay.

关 键 词：黄曲霉毒素 生物防治 拮抗物 HPLC 线性梯度洗脱 

分 类 号：TS201.3[轻工技术与工程—食品科学]