不同供氧方式对木醋杆菌培养中生物量的影响  

Effects of Different Oxygen-supply Modes on Biomass of Acetobacter xylinum

作　　者：向东[1] 王锡彬[1] 钟春燕 

机构地区：[1]海南大学食品学院,海南海口570228 [2]海南椰国食品有限公司,海南海口570311

出　　处：《现代食品科技》2011年第8期873-876,共4页Modern Food Science and Technology

基　　金：国家科技支撑计划项目(2007BAD76B01)

摘　　要：分别采用静置、底部供氧、间歇式供氧、改进供氧系统等4种培养方式,用10 L发酵罐培养木醋杆菌菌株HN001,研究供氧方式对木醋杆菌生物量的影响。结果表明,通过发酵罐培养木醋杆菌,生物量的变化并未随溶氧量增大而增大。不同供氧方式发酵液中的溶氧量不同,底部供氧方式溶氧量最大,但这种培养方式形成了较大的搅拌和剪切力,生产菌株容易产生负突变,木醋杆菌生物量最小;改进供氧系统培养方式通过发酵罐底部盘旋至顶部的硅胶管供氧,对培养液的搅拌和剪切力较小,培养7 d后,木醋杆菌生物量可达14.80×104 cfu/mL。比生产中常用的静置培养方式增加了近10倍,同时也优于底部供氧、间歇式供氧培养方式。Acetobacter xylinum strain named HN001 was cultivated in the 10 liter fermentation tank by different ways including stationary culture mode,bottom oxygen-supply mode,rhythmic oxygen-supply mode,and modified system of oxygen-supply mode.The results showed that different dissolved oxygen（DO） values in the fermentation liquid were obtained with different oxygen-supply modes but the biomass of HN001 strain showed no increase with increasing DO value.The maximal value of DO was obtained by the bottom oxygen-supply mode which leading to sharp stirs and shear force in the fermentation tank.However,the minimal biomass was achieved because the Acetobacter xylinum strain was liable to negative mutation in this oxygen-supply mode.A silicon tube circling from bottom to top of the tank was used to supply oxygen which formed the modified system of oxygen-supply and gained weak stirs and shear force and the biomass of Acetobacter xylinum strain reached to 14.80×104 viable counts/ml after a 7-day cultivation.The results showed that the modified system of oxygen-supply mode was the best culture mode and the biomass of strain increased nearly 10 times than stationary culture mode.

关 键 词：木醋杆菌 生物量 发酵罐 供氧 培养 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]