菜籽多糖WPS-1对小鼠腹腔巨噬细胞免疫功能的影响  

Immunomodulating Effects of Rapeseed Polysaccharide WPS-1 on Mouse Peritoneal Macrophage

作　　者：朱建飞[1,2] 唐春红[1,2] 吴谋成[3] 

机构地区：[1]重庆工商大学环境与生物工程学院,重庆400067 [2]重庆工商大学绿色食品研究所,重庆400067 [3]华中农业大学食品科学技术学院,武汉430070

出　　处：《中国粮油学报》2011年第8期41-44,49,共5页Journal of the Chinese Cereals and Oils Association

基　　金："十五"国家重大科技专项(200lBA501A20);"十一五"湖北省重大科技攻关项目(2006AA20lB32)

摘　　要：采用MTT法检测细胞的活性,菜籽多糖WPS-1能促进巨噬细胞增殖,并表现出明显的剂量-效果关系,WPS-1促进巨噬细胞增殖的最佳质量浓度为40μg/mL。采用半定量RT-PCR进一步研究不同作用时间下40μg/mL WPS-1对腹腔巨噬细胞IL-6、肿瘤坏死因子α(TNF-α)这两种细胞因子以及诱生型一氧化氮合酶(iNOS)mRNA表达的影响。WPS-1促进腹腔巨噬细胞IL-6、TNF-α和iNOS mRNA表达的最佳时间为24 h。菜籽多糖对巨噬细胞的免疫功能具有调节作用。The MTT assay was adopted to measure cell activation. Rapeseed polysaccharide WPS - 1 promoted proliferation macrophage cells in a dose - dependent manner. The optimal concentration of WPS - 1 to promote prolif- eration was 40 μg/mL. Semi - quantitative RT - PCR was performed to determine changes in cytokine mRNA expres- sion. The influence of WPS - 1 to IL - 6, tumor necrosis factor -α （ TNF -α） , and inducible nitric oxide synthase （iNOS） rnRNA expression were all 40 μg/mL. The best time of WPS - 1 to promote IL - 6, TNF -α, iNOS mRNA of expression were all 24 h. The results indicated that rapeseed polysaccharide could enhance the immune regulation of peritoneal macrophages.

关 键 词：菜籽多糖 巨噬细胞 免疫作用 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]