普通油茶种子4个发育时期的转录组分析  

Transcriptome Analysis of Camellia oleifera Abel Seed in Four Development Stages

作　　者：林萍[1] 曹永庆[1] 姚小华[1] 王开良[1] 滕建华 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]浙江省金华市婺城区东方红林场,金华321025

出　　处：《分子植物育种》2011年第4期498-505,共8页Molecular Plant Breeding

基　　金："十一五"科技支撑计划项目(2009BADB1B012009BADA8B04-06);中国林科院公益性科研基金专项(CAFYBB2008005CAFYBB2008011CAFYBB2008036);中国林科院亚林所公益性科研基金专项(RISF6804)共同资助

摘　　要：我国油茶产业迅速发展,种苗繁育及栽培技术不断进步,但油茶分子基础研究薄弱,决定油茶产量、茶油质量、抗性等指标的重要经济、生长性状的分子机理研究甚少,这必将阻碍油茶产业的可持续发展。本项目首次采用高通量测序技术solexa技术对普通油茶"长林4号"无性系种子发育的4个时期转录组进行测序,经组装分析获得80 310条unigene,其中确定编码蛋白功能的有21 789条,分为24大类,占All-unigene的27.13%。通过与KEGG库比对,对42 638个unigene进行了Pathway注释,涉及的pathway有265个,主要包括新陈代谢、遗传信息加工、细胞代谢等相关pathway。这些相关研究为分析基因表达的数量及调控模式,分析基因表达调控元件的变化规律,揭示不同发育期基因表达差异等研究打下了良好基础,将为定向育种提供有力的理论指导和技术支持。The oil tea industry is developing very fast in China,and great improvements have been made in plantlet propagation and culture technology.While,the molecular mechanisms on tea oil production,quality and resistance were not researched,and which will hamper the development of oil tea industry greatly.To investigate the profile of gene expression in Camellia oleifera and elucidate its functional gene,Solexa sequencing were used to produce a substantial expressed sequence tags dataset from the seed of ＂Changlin4＂.A total of 80 310 unigenes and 21 789 unigenes with protein function annotations were generated.The unigenes with function annotations can be classified into twenty-four functional-categories and account for 27.13% of all unigenes.The All-Unigene were queried against the KEGG pathway database,and 42 638 unigenes were given the pathway annotations and related to 265 pathways,including metabolism,genetic information processing,cell metabolism pathways and so on.Data presented in this study will constitute an important resource for the scientific community that is interested in the molecular genetics and functional genomics of Camellia oleifera.

关 键 词：油茶(Camellia oleifera) Solexa测序技术 UNIGENE PATHWAY 

分 类 号：S794.4[农业科学—林木遗传育种]