激光共聚焦扫描显微镜观察ERα和MTA1在乳腺癌细胞内的共定位  

Confocal laser scanning microscope for intracellular colocalization of ERα and MTA1 in breast cancer cells

作　　者：刘芙蓉[1] 李彦姝[1] 张红艳[1] 李丰[1] 

机构地区：[1]中国医科大学细胞生物学教研室卫生部细胞生物学重点实验室教育部医学细胞生物学重点实验室,辽宁沈阳110001

出　　处：《电子显微学报》2011年第3期244-249,共6页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金重大研究计划资助项目(No.90813038);国家自然科学基金资助项目(No.30771128)

摘　　要：目的:观察ERα和MTA1在乳腺癌细胞内的共定位。方法:在有无雌激素或拮抗剂刺激下,利用激光共聚焦扫描显微镜观察ERα和MTA1在乳腺癌细胞系MCF-7中的共定位。结果:在无雌激素刺激下两者共定位于细胞浆和细胞核,而在雌激素刺激后两者则共定位于细胞核内。在雌激素受体拮抗剂刺激下,两者在细胞浆内定位增加。结论:雌激素和雌激素受体拮抗剂刺激乳腺癌细胞改变ERα和MTA1的共定位。Objective： Observation of the colocalization of ERα and MTA1 in breast cancer cells.Methods： Observation of the colocalization of ERα and MTA1 in breast cancer cells using confocal laser scanning microscope without/with 17β-estradiol or its antagonist.Results： The two proteins were colocalized in the cytoplasm and nucleus without 17β-estradiol stimulation,while they were colocalized in the nucleus with the estrogen stimulation.Under the stimulation of the ER antagonist,the colocalization of the two proteins was increased in the cytoplasm.Conclusion： The changes of colocalization of ERα and MTA1 were influenced by estrogen and the ER antagonist in breast cancer.

关 键 词：ERΑ MTA1 共定位 激光共聚焦扫描显微镜 

分 类 号：R737.9[医药卫生—肿瘤]