葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用  

Protective Effects of Puerarin Combined with Taurine on Pancreas of Rats with Type 2 Diabetes

作　　者：门秀丽[1] 赵利军[1] 孔小燕[1] 赵霞[1] 李克宁[1] 张连元[1] 

机构地区：[1]河北联合大学基础医学院病理生理教研室,唐山063000

出　　处：《天津医药》2011年第8期730-732,共3页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(项目编号:30971410);河北省科技厅基金资助项目(项目编号:10276105D-84)

摘　　要：目的:探讨葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用。方法:将成模的40只Wistar大鼠随机分为糖尿病组(DM组)、单用牛磺酸组(Tau组)、单用葛根素组(Pue组)和葛根素联合牛磺酸组(Pue+Tau组)。8周后,测4组大鼠糖化血红蛋白(HbA1c)、血浆葡萄糖(Glucose)、胰岛素(Insulin)、超氧化物歧化酶(SOD)、丙二醛(MDA)、活性氧(ROS)及胰腺线粒体MDA、ROS及SOD,免疫组织化学方法检测各组大鼠胰腺组织胰十二指肠同源盒基因1(PDX-1)的表达。结果:Tau组、Pue组及Pue+Tau组较DM组大鼠血浆SOD活性和血浆胰岛素水平均有显著升高,而Glucose、MDA、ROS及HbA1c呈不同程度的降低;并且3个给药组大鼠胰腺线粒体MDA和ROS含量较DM组均有不同程度的降低,而SOD活性均有显著升高,其中Pue+Tau组这种变化更加明显;免疫组织化学染色显示DM组大鼠胰岛细胞中PDX-1表达水平较低,Tau和Pue组胰岛细胞PDX-1表达上调,Pue+Tau组PDX-1表达明显上调。结论:葛根素联合牛磺酸对2型糖尿病大鼠胰腺有保护作用,其机制可能与降低氧化应激水平,上调PDX-1基因表达有关。Objective：To investigate the protective effects of puerarin combined with taurine on pancreas of rats with type 2 diabetes.Methods：Forty Wistar rats with diabetes mellitus were randomly divided into diabetes mellitus group（DM）,taurine treatment group（Tau）,puerarin treatment group（Pue） and puerarin combined with taurine treatment group（Pue＋Tau）.After 8-week treatment,the serum levels of glycosylated hemoglobin（HbA1c）,glucose,insulin,malondialdehyde（MDA）,superoxide dismutase（SOD） and reactive oxygen species（ROS） were measured in rat groups.The activity of SOD,MDA and ROS in pancreatic mitochondria were detected respectively as well.Immunohistochemistry technique was used to detect the expression of pancreatic duodenal homeobox-1（PDX-1） in pancreas of different rat groups respectively.Results：Compared with DM group,the activity of SOD and the content of insulin were higher,but glucose,MDA,ROS and HbA1c were lower in Tau group,Pue group and Pue＋Tau group.Moreover the contents of MDA and ROS of pancreatic mitochondria were decreased,but the activity of SOD increased,in these three treatment groups than those in DM group.More significant changes were found in Pue＋Tau group.The expression of PDX-1 protein was significantly lower in DM group,but up-regulated in pancreatic islets in Pue＋Tau group compared with that of DM group.Conclusion：These results suggest that puerarin combined with taurine could prevent the pancreas from oxidative stress in diabetic rats,which is related with the up-regulation of PDX-1 expression.

关 键 词：糖尿病 2型 牛磺酸 葛根素 胰腺 血红蛋白A 糖基化 胰岛素 免疫组织化学 模型 动物 

分 类 号：R587.1[医药卫生—内分泌]