TRAIL联合Res对乳腺癌MDA-MB-231细胞DR和Caspase表达的影响  

The Expression of DR and Caspase in TRAIL Combined with Resveratrol Inducing Apoptosis of MDA-MB-231 Breast Cancer Cells

作　　者：李岩 赵丹[1] 刘新莉[1] 马萍[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所二室,辽宁沈阳110001

出　　处：《中国血液流变学杂志》2011年第2期222-226,共5页Chinese Journal of Hemorheology

摘　　要：目的研究TRAIL联合白藜芦醇（Res）对乳腺癌MDA-MB-231细胞DR和caspase表达的影响。方法MTT法检测细胞增殖；流式细胞术检测细胞凋亡及细胞表面DR4、DR5蛋白的表达；分光光度法检测caspase-3、caspase-8相对活性；荧光定量PCR检测DR4、DR5及caspase-3、caspase-8 mRNA的表达。结果50μmol／L和100μmo/L,Res分别与50ng／mL TRAIL联合作用后，对MDA-MB-231细胞增殖的抑制率及细胞凋亡率分别与单独应用相应浓度的Res和TRAIL比较，均存在显著差异（P〈0．01）。50μmol／L和100μmol／LRes分别与50ng／mLT RAIL联合作用后，caspase-3、caspase-8的相对活性与对照组及单用TRAIL、Res比较均明显增强，差异显著（P〈0．01）。Res作用后，细胞表面DR4、DR5荧光指数与对照组比较明显增强。荧光定量PCR结果显示与对照组比较．Res作用后DR4、DR5mRNA表达上调；与单独用药比较，TRAIL联合Resetcagpase-3、easpase-8 mRNA表达上调。结论TRAIL和Res联合应用对诱导乳腺癌MDA-MB-231细胞凋亡具有明显的协同效果，其作用机制可能与增加DR和caspase活性有关。Objective To explore the mechanism of TRAIL combined with resveratrol inducing apoptosis in MDA-MB-231 breast cancer cell line. Methods The proliferation was detected by MTT assay.Apoptosis of cells and the expression of DR4 and DR5 on the surface of MDA-MB-231 cell were examined by flow cytometry. The relative activity of caspase-3 and caspase-8 were examined by spectrophotometric method.The expression of DR4,DR5,caspase-3 and caspase-8 mRNA were measured by Real time reverse transcription-polymerase chain reaction（Real time PCR）.Results After cells were exposed to 50ng/mL TRAIL combined with 50 μmol/L,100 μmol/L resveratrol for 12h,the inhibitory rate and the apoptotic percentage were increased markedly（P 〈 0.01）.After cells were exposed to 50ng/mL TRAIL combined with 50 μmol/L,100 μmol/L resveratrol for 12h,the relative activities of caspase-3 and caspase-8 were advanced（P 〈 0.01）.Compared with control group and one treatment factor, the fluorescence index of DR4 and DR5 on the surface of cells were increased （P 〈 0.05）. And the expression of DR4,DR5,caspase-3 and caspase-8 mRNA were enhanced markedly（P 〈 0.0 l）.Conelusion TRAIL and resveratrol are able to kill breast cancer MDA-MB-231 cell,respectively, while TRAIL with resveratrol together applied can have synergistic effect on MDA-MB-231 cell by increasing the DR and caspase activities.

关 键 词：乳腺癌 凋亡 TRAIL 白藜芦醇 死亡受体 CASPASE 

分 类 号：R737.9[医药卫生—肿瘤]