胃癌微卫星不稳定性与移码突变的关系  

Microsatellite instability and frameshift mutation in gastric carcinoma

作　　者：房殿春[1] 罗元辉[1] 杨仕明[1] 刘为纹[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《中华消化杂志》1999年第6期385-387,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金

摘　　要：目的　研究胃癌微卫星不稳定（ ＭＳＩ） 与移码突变的关系。方法　采用ＰＣＲ为基础的方法检测了４４ 例胃癌８ 个微卫星位点和ｈＭＳＨ６ 、ＢＡＸ和ＴＧＦβＲⅡ移码突变。结果　胃癌ＭＳＩ的检出率为２５．０ ％ （１１／４４ 例） 。６ 例（１３ ．６％ ） 为高ＭＳＩ（４ 个以上位点），５ 例（１１ ．４ ％ ）为低ＭＳＩ（１ ～３ 个位点）。肠型胃癌高ＭＳＩ的检出率显著高于弥漫型胃癌（ Ｐ＜ ０．０５） 。４４ 例胃癌中检出ＴＧＦβＲⅡ移码突变４ 例，ＢＡＸ 和ｈＭＳＨ６ 基因移码突变各１ 例。以上移码突变均发生于高ＭＳＩ组，而低ＭＳＩ和ＭＳＩ阴性组未见有移码突变。结论　高ＭＳＩ胃癌有单核苷酸移码突变倾向。Objective To study the relationship between microsatellite instability (MSI) and frameshift mutations of hMSH6, BAX and TGFβ RⅡ. Methods Eight microsatellite loci and frameshift mutations of hMSH6, BAX and TGFβ RⅡ were analyzed by polymerase chain reaction (PCR) based methods. Results MSI was detected in 25.0% (11/44) of gastric cancer. Six (13.6%) gastric carcinomas were high MSI (Four Loci or morc) and five (11.4%) were low MSI (one to three loci). The frequency of MSI high proved to be significantly higher in intestinal type than in diffuse carcinomas ( P <0.05). Of the 44 gastric carcinomas, frameshift mutations were detected in 4 for TGFβ RⅡ, 1 for BAX and 1 for hMSH6. All frameshift mutations were detected in gastric carcinomas with high MSI, none was detected in those with low MSI or MSI negative. Conclusions Gastric cancers with high MSI exihibits a tendency to accumulate frameshift mutations in simple repeated nucleotide sequence.

关 键 词：胃肿瘤 微卫星不稳定性 移码突变 

分 类 号：R735.202[医药卫生—肿瘤]