罗伊乳杆菌转化甘油能力的原位再激活  

In Situ Reactivation of the Ability of Lactobacillus reuteri Converting Glycerol

作　　者：王珍珠[1] 陈国[1] 陈宏文[1] 

机构地区：[1]华侨大学化工学院,福建泉州362021

出　　处：《华侨大学学报（自然科学版）》2011年第4期414-417,共4页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金资助项目(20906035);福建省自然科学基金资助项目(E0810017)

摘　　要：考察罗伊氏乳杆菌(L.reuteri)静息细胞转化甘油的重复利用,甘油脱水酶的原位再激活,以及补加培养基对再激活甘油脱水酶的变化.结果表明:菌体经二次转化后基本丧失转化能力,甘油对胞内甘油脱水酶具有强烈的抑制灭活作用;确证胞内存在甘油脱水酶再激活体系;不具转化甘油能力的L.reuteri可被补加的培养基在一定程度上再激活,使其能够重复利用.In this paper,reusing of Lactobacillus reuteri resting cell,reactivation of Glycerol dehydratase in situ and reacting converting glycerol of Lactobacillus reuteri by adding fresh culture medium were studied.The results indicated that the Lactobacillus reuteri would lose its transformation ability after converting twice,glycerol had strong inhibitory activity to Glycerol dehydratase inside the cell and it was confirmed the cell owned a reactivating system for Glycerol dehydratase,and Lactobacillus reuteri which wasn′t able to convert glycerol could react to a certain extent by adding culture medium,and it could convert glycerol again.

关 键 词：罗伊氏乳杆菌 甘油脱水酶 原位再激活 静息细胞 甘油 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]