黑色素瘤抗原基因MAGE-A3 5′端CpG岛异常甲基化检测  

The 5′CpG island methylation patterns of melanoma antigen gene MAGE-A3

作　　者：毛恺[1] 丁肖华[2] 邱耕[3] 叶松山[3] 杨晓菲[3] 何蕴韶[3] 

机构地区：[1]河南省新乡市中心医院胸瘤二科,453000 [2]新乡医学院三全学院检验系,河南新乡453003 [3]中山大学达安基因科研部,广州510080

出　　处：《国际检验医学杂志》2011年第12期1294-1296,共3页International Journal of Laboratory Medicine

摘　　要：目的研究黑色素瘤抗原基因MAGE-A3 5′端CpG岛异常甲基化状态,探讨其作为肝细胞肝癌(HCC)肿瘤标志物的可行性。方法利用重亚硫酸盐测序聚合酶链反应(BSP)分析HCC细胞株HepG2和健康对照者来源白细胞基因组中MAGE-A3基因5′端CpG岛异常甲基化状态,寻找HCC细胞MAGE-A3 5′端CpG岛去甲基化位点。结果 HepG2细胞和健康对照者来源白细胞基因组MAGE-A3 5′端CpG岛存在不同的甲基化状态,前者存在特异性去甲基化位点。结论 MAGE-A3 5′端CpG岛去甲基化位点的检测可用于HCC的早期诊断。Objective To study the 5′CpG island methylation patterns of melanoma antigen gene MAGE-A3,and relative application as tumor marker of hepatocellular carcinoma（HCC）.Methods BSP was used for the detection of the methylation /demethylation status of CpG site among the 5′CpG island of MAGE-A3 in genomic DNA from HepG2 cells and white blood cells（WBC）of healthy controls to identify the HCC cells specific demethylation sites of CpG islands.Results Several CpG sites in MAGE-A3 promoters were found with different methylation patterns in HCC cells,as compared to those in WBC of healthy controls,and demethylation patterns of CpG island specific for HepG2 cells were indentified.Conclusion Demethylation pattern of MAGE-A3 5′CpG islands could be used as tumor marker for the early diagnosis of HCC.

关 键 词：肿瘤 肝细胞 去甲基化 

分 类 号：R739.5[医药卫生—肿瘤]