亚临界流体酶催化制备富含多不饱和脂肪酸的磷脂研究  

Study on enzymatic preparation of n-3 polyunsaturated fatty acidriched phospholipids in subcritical 1,1,1,2-tetrafluoroethane

作　　者：于刚[1] 张慧[1] 王玉明[2] 薛长湖[2] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《广东农业科学》2011年第14期1-5,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30972285);广东省海洋渔业科技推广专项(A200901Ⅰ03);中央级科研院所基本科研业务费专项(2010YD11)

摘　　要：以亚临界1,1,1,2-四氟乙烷(R134a)为介质,固定化脂肪酶Lipozym TL IM催化鱼油多不饱和脂肪酸与大豆磷脂的酸解反应制备EPA/DHA磷脂,探讨了压力(2～8 MPa)、温度(30～60℃)、水分、接触时间(1～12 h)对酶稳定性的影响,研究了固定化脂肪酶Lipozyme TL IM在亚临界R134a中催化大豆磷脂酸解的影响因素。结果表明,在试验选定的压力、温度、水分、接触时间范围内,固定化脂肪酶Lipozyme TL IM保持高稳定性,在各试验条件下酶活均呈现不同程度的提高,在4 MPa、40℃、处理7 h,1∶1(酶/水,W/W)的条件下,酶活残余率高达194.9%;固定化脂肪酶Lipozyme TL IM在亚临界R134a中表现出较高的催化活性,需要较少的酶量即可迅速达到较高的转化率,反应不存在底物抑制。在10%酶量、6 MPa、40℃、游离脂肪酸与磷脂的摩尔比10∶1、反应1 h的条件下,转化率达50.16%。In subcritical 1,1,1,2-tetrafluoroethane（R134a）,commercial immobilized lipase Lipozyme TL IM was used as catalyst for preparation of EPA/DHA phospholipids.The stability of Lipozyme TL IM in subcritical R134a was investigated.The results indicated that subcritical R134a treatment led to significant increase of activity of Lipozyme TL IM.No loss of activity of Lipozyme TL IM treated with subcritical R134a under different operation factors（pressure 2~8 MPa,temperature 30~60℃,incubation time 1~12 h,water content 1：1,1∶2,1∶5 enzyme/water） was observed.The influences of factors on Lipozyme TL IM-catalyzed acidolysis between soybean phospholipids and free fatty acids was investigated.These factors included amount of enzyme,pressure,temperature,water content and molar ratio of substrates.The results indicated that Lipozyme TL IM exhibited high activity in subcritical R134a and higher conversion was obtained with lower amount of enzyme.In subcritical R134a,6 MPa,40℃,reaction time 1 hour,enzyme amount 10% of substrates,substrate molar ratio 10：1（n-3 polyunsaturated fatty acid/ soybean phospholipids,mol/mol）,and conversion was 50.16% under these conditions.

关 键 词：酶催化 制备 磷脂 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]