超声波协同酶法提取大豆多糖工艺的研究  

Ultrasonic-Assisted Enzymatic Hydrolysis of Bean Dregs for Extraction of Polysaccharides

作　　者：陈红[1] 崔海月[1] 李玉扩[1] 刘秀奇[1] 樊红秀[1] 王大为[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118

出　　处：《吉林农业大学学报》2011年第5期581-586,共6页Journal of Jilin Agricultural University

基　　金：国家"863"计划项目(2007AA10Z336)

摘　　要：以豆渣为原料,采用超声波协同酶法提取大豆多糖并对其工艺进行了优化。通过单因素试验和正交试验,确定超声波协同酶法提取的最佳工艺条件:超声波功率200 W,超声波辐射时间30 min,料液质量浓度0.04 g/mL,纤维素酶用量1.5%,酶解温度50℃,酶解时间40 min,pH5.0,在此工艺条件下,多糖的得率为12.23%。The present study aimed at developing an optimal procedure for the extraction of polysaccharides from the bean dregs hydrolyzed with a commercial cellulase preparation under ultrasonic assistance.The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows： ultrasonic powder 200 W for 30 min treatment,material/water concentration is 0.04 g/mL,enzyme dosage 1.5%,pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min.Under such conditions,a maximum polysaccharides yield of up to 12.23% was obtained.

关 键 词：豆渣 多糖 超声波 纤维素酶 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]