胃癌中CDC4和cyclin E的表达及临床意义  

The Expression and Significance of CDC4 and cyclin E in Gastric Cancer

作　　者：黄国全[1] 张才全[1] 彭小超[1] 张能[1] 汤为学[2] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科 [2]重庆医科大学重庆市神经病学重点实验室,中国重庆400016

出　　处：《生命科学研究》2011年第4期339-344,共6页Life Science Research

摘　　要：检测胃癌中CDC4/Fbxw7、cyclin E的表达,分析其与胃癌临床病理特征的关系和临床意义.采用逆转录多聚酶链反应(RT-PCR)检测部分胃癌和胃正常组织中CDC4、cyclin E mRNA的表达;免疫组化(SP法)检测60例胃癌组织及对应正常组织中CDC4/FBXW7、cyclin E蛋白的表达,探讨两者的临床病理特征之间的关系.胃癌中CDC4蛋白表达显著低于正常组织(P<0.05),而cyclin E蛋白的表达在胃癌组织中显著增高,阳性率与癌旁正常组织比较,差异有统计学意义(P<0.01).CDC4、cyclin E蛋白和mRNA表达均与胃癌的分化程度、TNM分期、淋巴结转移及浸润深度有关.CDC4、cyclin E在mRNA表达水平上呈负相关.CDC4的表达缺失可能导致cyclin E的过表达.CDC4的低表达可能是胃癌诊疗及预后判断的重要生物学指标.To investigate the expression of CDC4 and cyclin E in gastric cancer and elucidate the potential relationship between their expression and clinical pathological characteristics. Semiquantitative reverse transcription polymerase chain reaction （RT-PCR） was determined the mRNA expression of CDC4 and cyclin E in part of random selecting gastric cancer samples and corresponding adjacent normal mucosal tissues; Im- munohistochemistry was used to detect the protein expression of CDC4 and cyclin E in 60 specimens of gastric cancers and corresponding adjacent normal mucosal tissues. The expression of CDC4, cyclin E and the clinical pathological characteristics were analyzed. The protein expression of CDC4 in gastric cancer tissues were significantly lower than that in adjacent normal mucosal tissues （P〈0.05）, whereas the protein expression of cyclin E in gastric cancer tissues were significantly higher than that in adjacent normal mucosal tissues （P〈0.01）. The protein and mRNA expression of CDC4, cyclin E were correlated with differentiation, TNM stage, lymph node metastasis, infiltration. There was a significant negative correlation between CDC4 and cyclin E at the mRNA expression levels, cyclin E overexpression is likely to be the CDC4/FBXW7 loss. The loss of CDC4 may be a valuable marker for assessing the diagnosis and treatment and the prognosis of gastric canzcer .

关 键 词：CDC4/FBXW7 CYCLIN E免疫组化 逆转录-聚合酶链反应(RT-PCR) 细胞周期调节基因 

分 类 号：R735.2[医药卫生—肿瘤]