EphA1基因在前列腺癌中的表达及临床意义  

Expression of EphA 1 in prostate cancers and its clinical significances

作　　者：王建东[1] 时姗姗[1] 杜军[1] 董迎春[1] 盛蓁[1] 周水根[2] 马恒辉[1] 陈洁宇[1] 周晓军[1] 

机构地区：[1]南京军区南京总医院病理科,南京210002 [2]南京军区南京总医院泌尿外科

出　　处：《中国男科学杂志》2011年第7期6-10,共5页Chinese Journal of Andrology

基　　金：国家自然基金资助项目（30970813,30930028）

摘　　要：目的检测EphA1基因在前列腺癌的表达，探讨其异常表达机制及其临床意义。方法利用RT—PCR检测EphA1在前列腺癌细胞系中的表达，并用重亚硫酸钠修饰的DNA直接测序法检测并分析其甲基化状态。免疫组织化学分析EphA1蛋白、p53蛋白、EGFR蛋白在前列腺癌组织中的表达及其相关性在临床中的意义。结果EphA1蛋白表达在前列腺癌和良性增生组织中差异有统计学意义（P=0．013），并且发现在前列腺癌细胞存在EphA1基因甲基化现象。EphA1蛋白表达上调与前列腺癌有较高的Gleason评分患者有相关性（P=0．017），并与EGFR阳性有相关性（P=0．056），EphA1蛋白下调与p53阳性有相关性（P=0．007）。结论EphA1甲基化可能是导致该基因下调的机制之一，在前列腺癌的发生、发展中可能具有重要作用。Objective To investigate the expression of EphA1 in prostate cancers and its clinical significance, as well as its pathological mechanism. Methods Expressions of EphA1, p53, EGFR protein were checked by immunohistochemistry. The mRNA expression of EphA1 was detected by RT-PCR. The status of CpG Island around translation start site of EphA1 in prostate cancer cell lines was analyzed by direct sequencing with the sodium bisulfate modification of DNA. Results There was a different expression in EphA1 protein between in prostate cancers tissues and in hyperplasia tissues （P=0.013）. Hypermethylation of CpG Island in EphA1 was found in PC-3 and 22RV1. Upregulation of EphA1 was frequently detected in prostate cancer patients with high Gleason score （P=0.017）, and with positive staining of EGFR （P=0.056）. Downregulation of EphA1 was frequently associated with patients with positive staining of p53 （P=0.007）. Conclusion Methylation might result in downregulation of EphA1 gene in prostate cancer tissues. It may have an important role in tumorigenesis and progression.

关 键 词：EphA1蛋白 P53蛋白 EGFR 前列腺癌 甲基化 

分 类 号：R737.25[医药卫生—肿瘤]