均匀设计法优化绿色木霉菌黄色素薄层色谱展开剂系统  

Optimization of the TLC Solvent System for Trichoderma Viride Yellow Pigment by the Uniform Experimential Design

作　　者：张晓毅[1,2] 赵美美[2] 于新[2] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽合肥230036 [2]仲恺农业工程学院轻工食品学院,广东广州510225

出　　处：《生物数学学报》2011年第2期371-376,共6页Journal of Biomathematics

基　　金：国家自然科学基金资助项目(30871765)

摘　　要：根据系统选择法确定了绿色木霉菌(Trichoderma viride)所产黄色素的薄层色谱展开剂组分,利用均匀设计实验及逐步回归法建立回归模型优化展开剂配比,最终得出优化的展开剂系统为为石油醚-乙酸乙酯-丙酮-乙酸=12.23:1:1.58:0.55,分离效果较理想,所得到的薄层色谱分离函数指标COF_2达到了42.09±0.79.According to the system selection method , determined TLC solvent system components of yellow pigment produced by the Trichoderma viride.Established regression model by the uniform design and quadratic polynomial stepwise regression to optimize the formula of TLC solvent system. The optimal formula solvent system is ： petroleum ether - ethyl acetate - acetone - acetic acid = 12.23： 1： 1.58： 0.55. It made the yellow pigment separated clearly and quantitated accurately in TLC.The function indicator COF2obtained by TLC separation reached 42.09±0.79.

关 键 词：绿色木霉菌黄色素 薄层色谱 展开剂系统优化 

分 类 号：O157.2[理学—数学] O658.1[理学—基础数学]