空心菜中提取叶绿素试验条件的研究  

RESEARCH ON EXTRACTION CONDITION OF CHLOROPHY FROM WATER SPINACH

作　　者：谢惠波[1] 曹家松[2] 龚红月[2] 黄莉[2] 陈平平[2] 夏鲜[2] 

机构地区：[1]泸州医学院公共卫生系营养与食品卫生教研室,四川泸州646000 [2]泸州医学院

出　　处：《泸州医学院学报》2011年第4期358-360,共3页Journal of Luzhou Medical College

基　　金：四川省科技厅资助项目(2006J13-147);四川省教育厅盗助项目(2005A072)

摘　　要：目的:以空心菜为原料,研究不同的原料处理方式,对叶绿素提取率的影响。方法:以紫外-分光光度法测定其吸光度作为评价提取条件的评判依据,根据Amon公式计算叶绿素的含量。结果:通过实验得到叶绿素的最佳提取条件是用烫漂晒干法干燥原料,经蒸馏水浸泡30min复水,用95%酒精为溶剂,在60℃水浴中搅拌提取2h。结论:空心菜富含天然绿色素,提取的色素稳定性较高,颜色鲜艳,是提取天然色素的理想原料。Objective：Using ipomoea aquatica forsk as raw material to research the effects of different processing on chlorophyⅡ extraction rate.Methods： Determined by ultraviolet-visible spectrophotomery,ChlorophyⅡ content was calculated with the formula of Amon.Results： The optimum extracting condition of ChlorophyⅡ from Ipomoea aquatica Forsk was shown as following： rinsed raw materials with boiling water and dried in the sun,doused in distilled water for 30 minutes,whisked in water bath of 60℃,extracted with 95% ethanol.Conclusion：Ipomoea aquatica is rich in natural green pigment.The pigment extracted form it have high stability and gay colours.So,ipomoea aquatica is a kind of ideal materal from which natural piginent extracted.

关 键 词：空心菜 叶绿素 提取 

分 类 号：R151.3[医药卫生—营养与食品卫生学]