高效液相色谱法分析马铃薯中α-茄碱  

Detection of α-Solanine in Potato by High Performance Liquid Chromatography

作　　者：肖文军[1,2] 李勤[1] 熊兴耀[1] 邹开静[2] 张盛[1,2] 陈凌[2] 任国谱[3] 

机构地区：[1]湖南农业大学国家植物功能成分工程技术研究中心,长沙410128 [2]湖南农业大学园艺园林学院植物资源工程系,长沙410128 [3]中南林业科技大学食品科学与工程学院,长沙410004

出　　处：《分析化学》2011年第9期1459-1460,共2页Chinese Journal of Analytical Chemistry

基　　金：国家"十二五"支撑计划(No.2011BAD10B00)资助项目

摘　　要：1引言α-茄碱是变绿发芽后的马铃薯中含量最高的一种生物碱,具有抗肿瘤、抑菌、强心、镇痛等[1]多种功效。目前对马铃薯中α-茄碱的高效液相色谱检测法（HPLC）报道较少,并存在重现性较差、目标峰难分开等问题。A method was established for the determination of α-solanine in potato by high performance liquid chromatography.The α-solanine was extracted from potato by refluxing extraction.The effects of different mobile phase composition,concentration,concentration ratio,flow speed,column temperature and other factors on α-solanine separation were studied.When the mobile phase was a mixture of acetonitrile and 0.4% phosphoric acid aqueous solution,flow speed was 0.8 mL/min,temperature was 30 ℃,detection wavelength was set at 210 nm,α-solanine could be separated well.The calibration of α-solanine was in good linearity in the range of 0.16-0.80 g/L.The average recovery was 96.3% with relative standard deviation of 1.3%（n=6）.

关 键 词：高效液相色谱法 生物碱 马铃薯 茄 抗肿瘤 检测法 重现性 

分 类 号：S532[农业科学—作物学] O657.72[理学—分析化学]