高速逆流色谱分离纯化红树莓果中的鞣花酸  

Isolation and Purification of Ellagic Acid from Raspberry by High-Speed Counter-Current Chromatography

作　　者：辛秀兰[1] 张东升[2] 陈亮[3] 王维彬[1] 张明隆[1] 

机构地区：[1]北京电子科技职业学院,北京100029 [2]国家林业局林产工业规划设计院,北京100013 [3]北京化工大学生命科学与技术学院,北京100029

出　　处：《食品研究与开发》2011年第8期65-67,共3页Food Research and Development

基　　金：公益性行业(农业)科研专项(201103037);科研基地-生物技术在制药和农产品加工中的应用平台建设;国家林业局林业产业化项目;现代林业林下产业升级关键技术(2130221)

摘　　要：建立高速逆流色谱分离纯化红树莓果中有效成分鞣花酸的新方法。两相溶剂系统为正丁醇∶异丙醇∶水(体积比)=2∶1∶3,上相为固定相,下相为流动相,流速为2 mL/min,转速为850 r/min,从120 g红树莓果中分离得到纯度为81.6%的鞣花酸35.2 mg。A method for isolation and purification of ellagic acid from raspberry by high-speed,Counter-Current Chromatography（HSCCC） was established.The two-phase solvent system was composed of n-butanol∶isopropanol∶water（2∶1∶3,Volume ratio）,with the upper phase as fixed phase,the lower phase as mobile phase,a flow rate at 2 mL/min and rotation speed at 850 r/min.As a result,35.2 mg ellagic acid was purified from 120 g raspberry and the purity was up to 81.6 %.

关 键 词：高速逆流色谱 红树莓 鞣花酸 

分 类 号：R284[医药卫生—中药学]