应用HPLC-ICP-MS联用技术进行砷形态分析样品前处理的研究  

Pretreatment of Arsenic Sample by High-Performance Liquid Chromatography-Inductively Coupled Plasma Mass Spectrometry

作　　者：周勇[1,2] 黄鹂[2] 刘玲铃[2] 王维洁[2] 

机构地区：[1]浙江海洋学院,浙江舟山316004 [2]国家海洋食品质量监督检验中心,浙江舟山316021

出　　处：《浙江国际海运职业技术学院学报》2011年第1期15-18,共4页Journal of Zhejiang International Maritime College

摘　　要：采用3种不同的前处理方式（25℃水提取、50%甲醇提取、5 mM四丁基氢氧化铵/3 mM草酸（pH6.5））对紫菜、铁钉菜、无沙海带进行砷提取,提取液经HPLC-DRC-ICP-MS测定。研究结果表明：3种水产品中大约25%-70%的总砷被提取,柱上回收率在70%-90%之间。对于紫菜样品,AsB仅被25℃水和5 mM四丁基氢氧化铵/3 mM草酸（pH6.5）提取,DMA是提取液中主要的砷形态;铁钉菜中砷含量较高,As（V）是其中主要的砷形态;无沙海带中主要是有机砷。Three extracting solutions were used to extract arsenic under the condition： 25℃ water,50% CH3OH and 5 mM C16H37NO/3 mM（COOH）2·2H2O（pH6.5） from seaweed,ishige okamurae and kelps without sands and the extracted liguid was detected by HPLC-DRC-ICP-MS.The results showed that ： about 25%-70% of the arsenic was extracted in three aquaticproducts,recoveries in column was between 70%-90%.To seaweed samples,AsB was extracted only by 25 ℃ water and 5mM C16H37NO/3 mM（COOH）2·2H2O（pH6.5）,DMA was the main form of extracted arsenic;there were more arsenic in ishige okamurae,As（V） was the major form of extracted arsenic.The organic arsenic was mainly extracted in the kelps without sand.

关 键 词：HPLC-ICP-MS 水产品 砷形态 

分 类 号：X131[环境科学与工程—环境科学]