马氏珠母贝高F值寡肽在Caco-2细胞中吸收的初步研究  

Preliminary Study on the Transport of Pinctada Martensii High Fischer Ratio Oligopeptides Across a Caco-2 monolayer

作　　者：郑惠娜[1] 章超桦[1] 吉宏武[1] 秦小明[1] 黄来珍[2] 

机构地区：[1]广东海洋大学食品科技学院,广东湛江524025 [2]广东海洋大学海洋药物研究与开发重点实验室,广东湛江524025

出　　处：《食品与生物技术学报》2011年第4期613-617,共5页Journal of Food Science and Biotechnology

基　　金：国家"十一五"支撑计划项目(2007BAD29B09);广东海洋大学引进人才基金项目(0912259)

摘　　要：采用Caco-2单层细胞模型体外模拟马氏珠母贝高F值寡肽(PHFP)小肠吸收,研究结果显示:无论是AP面→BL面还是BL面→AP面,PHFP的吸收量均呈增加趋势。添加P-糖蛋白的专属抑制剂维拉帕米后,AP→BL的渗透系数由原来的6.83×10-7cm/min提高到8.16×10-7cm/min,而BL→AP渗透系数由8.84×10-7cm/min降低到2.20 10-7cm/min,说明PHFP溶液在Caco-2细胞中的转运存在外排泵的作用。并且,PHFP在Caco-2细胞转运过程中被细胞中所存在的代谢酶所代谢,而添加维拉帕米后,PHFP的代谢量有所降低。Based on the sequence alignment of Bacillus amyloliquefaciens,two pairs of primers were designed and synthesized in this study.Using the total DNA of Bacillus amyloliquefaciens as template,the gene of β-1,3-1,4-glucanase（bgl） was amplified by PCR,which has about 750 bp in length.The gene was cloned into pTG19-T Easy vector,and the recombinant plasmid was identified by PCR,restriction enzyme analysis and sequencing.The homology analysis revealed that the nucleotide sequences of the cloned β-1,3-1,4-glucanase gene similar to the B.amyloliquefacines（M15674）,B.subtilis（D00518） and B.licheniformis（AY365256）were 99%,95% and 94%,respectively.

关 键 词：马氏珠母贝 高F值寡肽 CACO-2细胞 

分 类 号：S917.4[农业科学—水产科学]