Identification and Expression of a b-actin Gene from Liposcelis bostrychophila Badonnel(Psocoptera:Liposcelididae)  

作　　者：JIANG Hong-bo SHEN Guang-mao DOU Wei TANG Pei-an LIU Yong-hua ZHOU An-wei WANG Jin-jun 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, P. R. China [2]College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, P.R. China

出　　处：《Agricultural Sciences in China》2011年第9期1391-1401,共11页中国农业科学（英文版）

基　　金：funded in part by the National Natural Sciences Foundation of China (30871631,31000860);the Specialized Research Fund for the Doctoral Program of Higher Education, China(200806350009) ;the Science and Technology Innovation Foundation for Graduate Students (kb2008001) of Southwest University, China

摘　　要：A β-actin gene, Libβ-actinl, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed in Escherichia coll. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding 376 amino acids with a predicted molecular weight of 41.82 kDa. According to a B lastN search, the coding region shared the highest identity （97%） with Pediculus hurnanus actin 5C, while the deduced amino acid sequence was completely identical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acid sequences confirmed the high similarity between Libβ-actinl and homologs in other insect species. The 3＇ untranslated region （3＇ UTR） of the Libβ-actinl mRNA had a high A＋U content （approximately 75%） and contained three repeats of the AUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Libβ-actinl was further analyzed in insecticide induced and control psocids. The results indicated that there was no significant difference in expression of Libβ-actinl between the induced and control groups, suggesting that Libβ-actinl may be an appropriate internal control for the gene expression profiling in this insect. Furthermore, Libβ-actinl was also heterologously expressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in the psocid.A β-actin gene, Libβ-actinl, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed in Escherichia coll. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding 376 amino acids with a predicted molecular weight of 41.82 kDa. According to a B lastN search, the coding region shared the highest identity （97%） with Pediculus hurnanus actin 5C, while the deduced amino acid sequence was completely identical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acid sequences confirmed the high similarity between Libβ-actinl and homologs in other insect species. The 3＇ untranslated region （3＇ UTR） of the Libβ-actinl mRNA had a high A＋U content （approximately 75%） and contained three repeats of the AUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Libβ-actinl was further analyzed in insecticide induced and control psocids. The results indicated that there was no significant difference in expression of Libβ-actinl between the induced and control groups, suggesting that Libβ-actinl may be an appropriate internal control for the gene expression profiling in this insect. Furthermore, Libβ-actinl was also heterologously expressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in the psocid.

关 键 词：ACTIN sequence analysis EXPRESSION real-time PCR 

分 类 号：Q959.226.4[生物学—动物学] S642.01[农业科学—蔬菜学]