化学发光酶免疫法检测玉米中脱氧雪腐镰刀菌烯醇  

Chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in corn

作　　者：楚金申[1] 许杨[1] 何庆华[1] 卢江[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室中德联合研究院,江西南昌330047

出　　处：《食品工业科技》2011年第9期407-410,共4页Science and Technology of Food Industry

基　　金："国家高技术研究发展计划"项目资助(2007AA10Z427)

摘　　要：应用抗脱氧镰刀菌烯醇单克隆抗体,以辣根过氧化物酶催化鲁米诺-过氧化氢化学发光体系,建立了一种简便、快速、高特异性的间接竞争化学发光酶免疫法用于检测谷物中的脱氧雪腐镰刀菌烯醇。所建立的方法分析灵敏度为10.7ng/mL,批内变异系数小于8.1%,批间变异系数小于12.8%,玉米的加标回收率为91.2%～99.5%,与其他常见真菌毒素未见明显交叉,所建立的标准曲线相关系数为0.9902,检测线性范围为12.9～163.7ng/mL,检测时间为40min。A sensitive indirect competitive chemiluminescence＇s enzyme immunoassay（CLEIA）for deoxynivalenol in corn was developed. The optimized CLEIA gave a limit detection of 10. 7ng/mL and a detection range of 12. 9～163. 7ng/mL,with an IC50 of 46. 01ng/mL and it was about 4. 01 times more sensitive than that of colorimetric ELISA. It had been validated on cereals samples in terms of precision（intra-and interassay coefficient variations of less than 8. 1% and 12. 8%,respectively）and accuracy（mean recovery 91. 2%～99. 5%）. These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of DON in corn samples.

关 键 词：脱氧雪腐镰刀菌烯醇(DON) 间接竞争化学发光酶免疫法(CLEIA) 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]