LAMP技术检测食品中产肠毒素大肠埃希氏菌  

Detection of Enterotoxigenic Escherichia coli in food by LAMP

作　　者：孟兆祥[1] 马晓燕[1] 张会彦[1] 张伟[1] 

机构地区：[1]河北农业大学食品科技学院,保定071001

出　　处：《食品科技》2011年第9期340-342,346,共4页Food Science and Technology

基　　金：河北省自然科学基金项目(C2008000216)

摘　　要：产肠毒素大肠埃希氏菌(Enterotoxigenic Escherichia coli,ETEC)是一种重要的引起人畜共患病的致病菌,能引起水样便及酸中毒,建立快速、准确的检测方法对预防和控制产肠毒素大肠埃希氏菌的感染具有重要意义。基于ETEC不耐热肠毒素LT基因序列,设计LAMP引物检测产肠毒素大肠埃希氏菌。结果表明:LAMP检测产肠毒素大肠埃希氏菌的检出限为32.9cfu/mL,人工污染生猪肉的检出限为55cfu/g。LAMP将可以成为替代PCR的核酸扩增新技术,为快速检测产肠毒素大肠埃希氏菌构建了一个技术平台。Enterotoxigenic Escherichia Coli （ETEC） is an important pathogen of zoonose, which can cause watery stools and acidosis. Thus, the development of a technique that can identify ETEC quickly and accurately is of great importance. LAMP primers were designed according to highly conserved heat-labile enterotoxin LT gene of Enterotoxigenic Escherichia Coli. The results show that the detection sensitivity of the LAMP method for Enterotoxigenic Escherichia Coli purely cultured was 32.9 cfu/mL and for Enterotoxigenic Escherichia Coli in contaminated meat was 55 cfu/g. LAMP has the potential to replace PCR, which can build a technology platform for the rapid detection of food-borne pathogens.

关 键 词：环介导等温扩增 产肠毒素大肠埃希氏菌(ETEC) 不耐热肠毒素(LT)基因 

分 类 号：TS207.4[轻工技术与工程—食品科学]